Glycosaminoglycans

Methods and Protocols
Herausgegeben:Balagurunathan, Kuberan; Nakato, Hiroshi; Desai, Umesh; Saijoh, Yukio

• Broschiertes Buch

Produktbeschreibung

This second edition volume expands on the previous edition with new information on the structural analysis of glycosaminoglycans (GAGs); chemical and enzymatic synthesis of GAGs; biophysical, biochemical, and computational analysis of GAG-protein interactions; molecular and genetic approaches to manipulating GAG cell biology; and molecular and genetic methods involving animal models and organoids. This volume also offers insight and guidance to non-glycoscience researchers who are performing advanced experiments on GAGs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Glycosaminoglycans: Methods and Protocols, Second Edition is a valuable experimental manual for practicing researchersincluding graduate and post-doctoral fellows, and provides them with the latest methodologies and practical tips to overcoming barriers in understanding the chemistry and biology of GAGs.

Produktdetails

• Methods in Molecular Biology 2303
• Verlag: Humana / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-0716-1400-6
• 2. Aufl.
• Seitenzahl: 852
• Erscheinungstermin: 10. Oktober 2022
• Englisch
• Abmessung: 254mm x 178mm x 46mm
• Gewicht: 1569g
• ISBN-13: 9781071614006
• ISBN-10: 1071614002
• Artikelnr.: 65705915

Inhaltsangabe

One-Pot Enzymatic Synthesis of Heparin from N-Sulfoheparosan.- Solution NMR Spectroscopy for Characterizing Protein-Glycosaminoglycan Interactions.- Metabolic Labeling of Proteoglycans and Analysis of Their Synthesis and Sorting in Filter-Grown and Polarized Epithelial Cells.- Fluorous-Tag Assisted Synthesis of GAG-Like Oligosaccharides.- Aqueous Molecular Dynamics for Understanding Glycosaminoglycan Recognition by Proteins.- A Non-Radioactive Method to Measure Hyaluronan Synthase Activity.- A Glycoproteomic Approach to Identify Novel Proteoglycans.- Analysis of Protein-Glycosaminoglycan Interactions using Traveling Wave Ion Mobility Mass Spectrometry.- Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical, and Spectroscopic Analysis.- Quantitative Disaccharide Profiling of Glycosaminoglycans from Two Different Preparations by PMP and Deuterated PMP Labeling.- Preparation and Characterization of Heparan Sulfate Derived Oligosaccharides to Investigate Protein-GAG Interaction and HS Biosynthesis Enzyme Activity.- Heparan Sulfate Structure: Methods to Study N-sulfation and NDST Action.- Methods for the Production of Recombinant Heparosan: A Critical Heparin Precursor, from Non-Pathogenic E.coli Strains.- De novo Sequencing of Heparin/Heparan Sulfate Oligosaccharides by Chemical Derivatization and LC-MS/MS.- Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans.- Nuclear Magnetic Resonance Methods in Structural Characterization of Glycosaminoglycans.- Aggrecan: Approaches to Study Biophysical and Biomechanical Properties.- Chromatographic Molecular Weight Measurements for Heparin: Its Fragments and Fractions, and Other Glycosaminoglycans.- Mass Spectrometric Methods for the Analysis of Heparin and Heparan Sulfate.- Affinity Purification of Glycosylphosphatidylinositol-Anchored Proteins by Alpha-Toxin.- Thermodynamic Affinity and Nature of Forces Defining Glycosaminoglycan: Protein Systems Using Fluorescence Spectroscopy.- Isolation and Characterization of Heparan Sulfate Containing Amyloid Precursor Protein Degradation Products.- Chemical Approaches to Prepare Modified Heparin and Heparosan Polymers for Biological Studies.- Glycosaminoglycan Quality Control by Monosaccharide Analysis.- Characterizing Thermodynamics of Protein-Glycosaminoglycan Interactions using Isothermal Titration Calorimetry.- Automated Synthesis of Chondroitin Sulfate Oligosaccharides.- Capillary Electrophoretic Analysis of Isolated Sulfated Polysaccharides to Characterize Pharmaceutical Products.- Preparation of Isotope Enriched Heparan Sulfate Precursors for Structural Biology Studies.- Methods for Measuring Exchangeable Protons in Glycosaminoglycans.- Advances in Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis.- anexVis: ATranscriptome Tool to Visualize Organ/Tissue-Specific Glycosaminoglycan Biosynthetic and Catabolic Pathways in Human Health and Diseases.- Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila.- Measuring Sulfatase Expression and Invasion in Glioblastoma.- Analyzing the Role of Heparan Sulfate Proteoglycans in Axon Guidance In Vivo in Zebrafish.- Regulation of 3-O-Sulfation of Heparan Sulfate during Transition from the Naïve to the Primed State in Mouse Embryonic Stem Cells.- Analysis of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility in Myofibroblast Differentiation.- Methods to Analyze the Effect of Diet-Derived Metabolites on Endothelial Inflammation and Cell Surface Glycosaminoglycans.- Assays for Evaluation of Substrates for and Inhibitors of beta-1,4-Galactosyltransferase 7.- Enzymatic Alteration of ECM to Explore Muscle Function and Motor Control of a Learned Behavior.- Procedures to Evaluate the Role of Heparan Sulfate in the Reactivity of Resistance and Conductance Arteries Ex Vivo.- Computerized Molecular Modeling for Discovering Promising Glycosaminoglycan Oligosaccharides that Modulate Protein Function.- Imaging Glycosaminoglycan Modification Patterns In Vivo.- Isolation and Purification of Versican and Analysis of Versican Proteolysis.- Identification of Cell Autonomous and Non-Cell Autonomous Functions of Heparan Sulfate Glycosaminoglycan Chains by Creating Chimeric Mouse Embryos.- Methods for Assessing the Effects of Xylosides on Angiogenesis.- Role of HSPGs in Systemic Bacterial Infections.- Generation of Drosophila Heparan Sulfate Mutant Cell Lines from Existing Fly Strains.- Application of a Mutant Cell Library to Determine the Structure-Function Relationship of Heparan Sulfate in Facilitating FGF-FGFR Signaling.- Manipulation of Glycosaminoglycans using Synthetic Xylosides toStudy their Roles in Lung Branching Morphogenesis in Ex Vivo Lung Bud Culture System.- Informatics Ecosystems to Advance the Biology of Glycans.- Analysis of 3'-phosphoadenosine 5'-phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse Transcription Polymerase Chain Reaction, and Immunohistochemistry.- Preparation of Nanosensors for Detecting the Activity of Glycosaminoglycan Cleaving Enzymes.- The Detection of Glycosaminoglycans in Pancreatic Islets and Lymphoid Tissues.- Histochemical Analysis of Heparan Sulfate 3-O-sulfotransferase Expression in Mouse Brain.- Mapping Proteoglycan Functions Using Novel Genetic Strategies.- Xyloside Derivatives as Molecular Tools to Selectively Inhibit Heparan Sulfate and Chondroitin Sulfate Proteoglycan Biosynthesis.- A Strategic Approach to Identification of Selective Inhibitors of Cancer Stem Cells.- Applications of Xylosides in the Manipulation of Stem Cell Niche to Regulate Human Neural Stem Cell Differentiation and Neurite Outgrowth.- Murine Models in the Evaluation of Heparan Sulfate-Based Anticoagulants.- Investigating the Roles of Heparan Sulfate Structures in Alpha-Synuclein Aggregation in Cell Culture Models.