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Sustainability is the overall goal in the management of grassland resources; it further determines the institutional framework within each grassland use is planned and implemented. Recent developments emphasize the need to include ecosystem services (supporting, provisioning, regulating and cultural) provided by grasslands into this framework. This reprint focuses on research advantages related to the ecology and management of grasslands and to the safeguarding of related ecosystem services, and provides an outline for future directions for sustainability. The reprint contains experimental studies and systematic reviews regarding grassland biodiversity, issues and threats related to grasslands, sustainable use of grasslands, ecosystem services they provide and other topics.