This book comprises a collection of chapters on the effect of plant growth regulators and Biofertilizer on growth and development of Sesame. The book looks at ways to establish long term safe, cost-effective and sustainable farming practices with greener methods. The book looks at the lead to useful guidance for applying PGRs and Biofertilizer to produce better growth of plants, especially for sesame. The book contains six chapters. Chapter 1 provides a general introduction of sesame plants and its necessity for growth enhancement. In chapter 2, a detailed description of Plant Growth Regulators and Biofertilizer is given. Chapter 3 provides the effect of PGRs on the growth and development of plants. In chapter 4, Biofertilizer and its effect on sesame growth were discussed. The effect of biochemical composition with respect to PGRs and Biofertilizer treatments is well explained in chapter 5. The greener approaches and future prospective of greener fertilizer application was concluded in chapter 6. Finally provide references.