A plant-mediated, green method of synthesizing copper and gold nanoparticle was successfully performed by employing the bark extract of Mitragyaa parvifolia and Adina Cordifolia. It was found out that the various biomolecules present in the bark extract were responsible for the formation and stability of the copper and gold nanoparticles. The CuNPs and AuNPs size, morphology, crystalline structure, and the stability were characterized by UV-Visible spectroscopy, scanning electron microscopy, X-ray diffraction, SEM and TEM analysis respectively. An effective antimicrobial activity of synthesized copper and gold nanoparticles against gram-positive (Basillus subtilis) and gram-negative (Escherichia coli) bacteria is observed. The potential in vitro anticancer activity of gold nanoparticles is observed against the MFC-7 cell line. In conclusion to be found effective drugs for the antibacterial activity and breast cancer.