Nanotechnology is being developed at several levels: materials, devices and systems. The non-material level is the most advancing at present, both in scientific knowledge and in commercial applications. In recent years nano-materials, specifically metal nano-particles, have received particular interest in diverse field of applied science ranging from material science to biotechnology. Biosynthesis of Gold Nano-Particles (AuNPs) as antimicrobial agent carried out by using the aqueous extract of fenugreek seeds that is Cost effective and environmentally friendly. This study targets Researcher, Scientist and Pharmacist in the Fields Nano biotechnology, Pharmaceutical and Chemical Engineering, Drugs Developing, Medicinal Plants, Pharmaceutical Microbiology.