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Research Paper (postgraduate) from the year 2019 in the subject Medicine - Pharmacology, Pharmacy, grade: 9.5, , language: English, abstract: In this study, we report the synthesis of Ag NPs by green synthesis process using Datura metel leaf extract as reducing agent. The final products characteristics were studied ¿ optical, structural, surface morphology and elemental analysis. Silver Nanoparticle has been recognized as a safe inorganic, antibacterial, nontoxic agent. Conventional synthesis of Silver Nanoparticle can generate toxic waste during the reaction process hence, this paper the green synthesis approach used and for synthesis of silver nanoparticle by using Datura metel leaf extract as a reducing agent. The change in colour of silver nitrate solution from colourless to brown was visual identification for formation of Silver Nanoparticle and shown the absorption at 447 nm. The synthesized Silver Nanoparticle were of size 180 nm analyse by SEM and further characetrised by FTIR where carboxylic peak was obtained at 1675 cm-1 as well as presence of alcoholic group and polyphenols. Further validated method was developed and linearity was found to be 0.995 and % RSD of validation parameters were found to be within the limit <2%. A simple, lab scale, rapid, accurate, precised and sensitive spectroscopic method has been developed and validated as per ICH guideline for synthesized silver nanoparticle in bulk pharmaceuticals by UV vis spectrophotoscopy and which is applicable for routine analysis.
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