Main description:
This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.
Key - Comprehensive protocols compiled specifically for this volume
- Among the topics covered:
- Genomic cloning
- Preparation and characterization of mRNA
cDNA cloning
- Screening libraries
- Identification and characterization of clones
- Methods written by 87 experts
- Editors' notes, overviews, and extensive cross-referencing
- Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility
- A problem-solving quantitative approach
- Emphasis on strategies for choosing alternative methods
Review quote:
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY
This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.
Key - Comprehensive protocols compiled specifically for this volume
- Among the topics covered:
- Genomic cloning
- Preparation and characterization of mRNA
cDNA cloning
- Screening libraries
- Identification and characterization of clones
- Methods written by 87 experts
- Editors' notes, overviews, and extensive cross-referencing
- Process Guide, a compendium of basic processes in molecular biology indexed for at-a-glance accessibility
- A problem-solving quantitative approach
- Emphasis on strategies for choosing alternative methods
Review quote:
Praise for the Series
"The Methods in Enzymology series represents the gold-standard."
--NEUROSCIENCE
"Incomparably useful."
--ANALYTICAL BIOCHEMISTRY
"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page."
--BIO/TECHNOLOGY
"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection."
--CHEMISTRY IN INDUSTRY
"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced."
--AMERICAN SOCIETY OF MICROBIOLOGY NEWS
"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."
--ENZYMOLOGIA
"A series that has established itself as a definitive reference for biochemists."
--JOURNAL OF CHROMATOGRAPHY