Main description:
The Guide to Protein Purification is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher.
Key - Contains procedures newly written for this volume
- Provides extensive practical information
- Clearly describes rationale and strategies for protein and enzyme purification
- Offers personal perspectives on enzyme purification by eminent researchers
Review quote:
"In summary, this is possibly the most important, comprehensive and affordable work on protein purification to have appeared in recent years."
TIB-TECH
Table of contents:
Developing Purification Procedures.
General Methods for Handling Proteins and Enzymes.
Optimization of Starting Materials.
Preparation of Extracts and Subcellular Fractionation.
Solubilization Procedures.
Purification Procedures: Bulk Methods.
Purification Procedures: Chromatographic Methods.
Purification Procedures: Electrophoretic Methods.
Specialized Purification Procedures.
Characterization of Purified Proteins.
Immunological Procedures.
Additional Techniques.
Concluding Remarks.
Personal Perspectives on Enzyme Purification.
Index.
The Guide to Protein Purification is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher.
Key - Contains procedures newly written for this volume
- Provides extensive practical information
- Clearly describes rationale and strategies for protein and enzyme purification
- Offers personal perspectives on enzyme purification by eminent researchers
Review quote:
"In summary, this is possibly the most important, comprehensive and affordable work on protein purification to have appeared in recent years."
TIB-TECH
Table of contents:
Developing Purification Procedures.
General Methods for Handling Proteins and Enzymes.
Optimization of Starting Materials.
Preparation of Extracts and Subcellular Fractionation.
Solubilization Procedures.
Purification Procedures: Bulk Methods.
Purification Procedures: Chromatographic Methods.
Purification Procedures: Electrophoretic Methods.
Specialized Purification Procedures.
Characterization of Purified Proteins.
Immunological Procedures.
Additional Techniques.
Concluding Remarks.
Personal Perspectives on Enzyme Purification.
Index.