Role stress among professionals is emerging as a highly discussed issue everywhere in the world from last few years of cut throat industrial competition. The relationship between each of the three gunas (sattva, rajas and tamas) mentioned as fundamental constituent of universal matter in ancient Indian Philosophy and various role stressors was established and the relationship between the dominant guna and the workplace stress experienced by the managers was analysed and interpreted. Findings show Informative results that the managers high on sattva guna were feeling more stress due to heavy workload, as comparatively higher duties were assigned to them observing them being more responsible than others. Meaningful interpretation of analysed facts clearly attributed relationship between role stress experienced by an individual at workplace and the Guna dominant in that individual. Research is of vital importance in terms of improving workplace behaviour of employees & enhancing quality of work life through managing an adequate balance of the three modes of material nature.