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Tight hamstrings are unpleasant to suffer with. They can make the backs of your legs ache after a day of sitting down, and you can often feel them pulling tight as you do simple things, like standing up. Hamstrings that are stretched tight like an extended elastic band can be prone to spraining, straining or even seriously tearing when placed under stress. Physiotherapy techniques like heat therapy, stretching exercises and Myofascial release plays important role in treatment of Hamstring tightness. Instrument assisted soft tissue mobilization or simply IASTM is a new range of tool which enables clinicians to efficiently locate and treat individuals diagnosed with soft tissue dysfunction. This project was undertaken to check the effectiveness of IASTM in cases with Hamstring muscle tightness. The instrument has given excellent results on muscle flexibility. Authors has tried to explain through this project evidence based use of IASTM on hamstring tightness.