This book provides fundamental knowledge of principal aspects of the experimental pharmacology right from laboratory animals handling and tissue mounting to practical implications of various important complex experimental procedures. This book is prepared using simple language and tried to explain the experiment with a model data of observation calculation. This book helps to reduce the gap between hands on experiment is not satisfactory learned by observing the effect of the drug in simulated experiment in pharmacology. The experimental methodology and experimental data explained. In this book are designed on the robust scientific experiment personalities. This book will be helpful for graduate and postgraduate related to pharmacology trainee, research workers during their day to day activity.