Main description:
Classical histology has been augmented by
immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method.
- The only book available that translates molecular genetics into cancer diagnosis
- Methods were developed by internationally-recognized experts and presented in step-by-step manner
- Results of each Immunohistochemical and in situ hybridization are presented in the form of color illustrations
Review quote:
The basics of cell biology and molecular biology precede each section that describes defects in genetic/epigenetic control. Gaining an understanding of molecular genetic defects in cancer, authors then proceed to tell you how to measure the defects, which reinforces your understanding of the basic pathobiologic process. The latest therapies are discussed in the context of how to repair these molecular defects. Timely chapters on specific molecular topics in ovarian and gastrointestinal tumors are presented in the format of formal research papers. Extremely well written and succinct, a perfect marriage of information for students at any level, from medical student to professors of oncology.
-- David J. Dabbs, M.D., Chief of Pathology, Magee Womens Hospital University of Pittsburgh School of Medicine
Table of contents:
PART I. MOLECULAR GENETICS
IDENTIFICATION OF TUMOR-SPECIFIC GENES
THE POSTTRANSLATIONAL PHASE OF GENE EXPRESSION IN TUMOR DIAGNOSIS
ROLE OF BARD1 IN APOPTOSIS AND CANCER
ANGIOGENESIS, METASTASIS AND EPIGENETICS IN CANCER
CAN EFFECTOR CELLS REALLY EFFECT AN ANTITUMOR RESPONSE AS CANCER THERAPY?
CIRCULATING CANCER CELLS
CIRCULATING CANCER CELLS: FLOW CYTOMETRY, VIDEO-MICROSCOPY AND CONFOCAL LASER SCANNING MICROSCOPY
PART 2. GASTROINTESTINAL CARCINOMA
GASTROINTESTINAL CARCINOMA: AN INTRODUCTION ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF P53 IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF p53, Rb, AND p16 PROTEINS IN ANAL SQUAMOUS CELL CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF p53 AND VEGF IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF p150 IN
GASTRIC CARCINOMA: THE ASSOCIATION WITH P53, APOPTOSIS AND CELL PROLIFARATION
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF Ki-67 IN ADENOCARCINOMA OF LARGE INTESTINE
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF KIT/CD117 IN GASTROINTESTINAL STROMAL TUMORS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF BUB1 PROTEIN IN GASTRIC CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF EPIDERMAL GROWTH FACTOR IN GASTRIC TUMORS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF BETA-CATENIN AND MUCIN IN STOMACH CANCER
LOSS OF CYCLIN D2 EXPRESSION IN GASTRIC CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF E-CADHERIN DIFFUSE-TYPE GASTRIC CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF APC AND E-CADHERIN IN GASTRIC CARCINOMA
IMMUNOHISTOCHEMICAL EXPRESSION OF CHROMOGRANIN A (CgA) AND Leu-7 IN GASTROINTESTINAL CARCINOIDS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF MUC5B IN GASTRIC CARCINOMA
ANGIOGENIN IN GASTRIC CANCER AND ITS ROLES IN MALIGNANCY
ROLE OF HELICOBACTOR PYLORI IN GASTRIC CANCER
PROTEIN ALTERATIONS IN GASTRIC ADENOCARCINOMA
VESICLE PROTEINS IN NEUROENDOCRINE AND NONENDOCRINE TUMORS OF THE GASTROINTESTINAL TRACT
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF CASPASE-3 IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF PRL-3 PHOSPHATASE IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF DNA METHYLTRANSFERASE 1 PROTEIN IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
CYTOPLASMIC TREFOIL FACTOR FAMILY-2 IN GASTRIC CANCER
PART 3. OVARIAN CARCINOMA
OVARIAN CARCINOMA: AN ITNRODUCTION
METHODS FOR DETECTING GENETIC ABNORMALITIES IN OVARIAN CARCINOMA USING FLUORSCENCE IN SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY
QUANTITATIVE IMMUNOHISTOCHEMISTRY (Q-IHC) BY
DETERMINING THE NORM OF THE IMAGE DATA FILE
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF HER2/neu IN HIGH GRADE OVARIAN SEROUS PAPILLARY CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF BRCA1 IN OVARIAN CARCINOMA
ROLE OF BRCA/BRCA2 IN OVARIAN, FALLOPIAN TUBE AND PERITONEAL PAPILLARY SEROUS CARCINOMA
K-RAS MUTATIONS IN SEROUS BORDERLINE TUMORS OF THE OVARY
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF Ki-67 IN OVARIAN CARCINOMA
THE ROLE OF EXPRESSION OF ESTROGEN RECEPTOR BETA,
PROLIFERATING CELL NUCLEAR ANTIGEN AND P53 IN OVARIAN GRANULOSA CELL TUMORS
ROLE OF CYCLOOXYGENASE-2 EXPRESSION
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
CYCLOOXYGENASE-2 AND PEROXISOME PROLIFERATOR ACTIVATED-RECEPTOR IN EPITHELIAL OVARAIN TUMORS
CDX-2 IMMUNOSTAINING IN PRIMARY AND SECONDARY OVARIAN CARCINOMAS
EPITHELIAL OVARIAN CANCER AND THE E-CADHERIN-CATENIN COMPLEX
ROLE OF CYTOKERATIN IMMUNOHISTOCHEMISTRY IN THE DIFFERENTIAL DIAGNOSIS OF OVARIAN TUMORS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
CYTOKERATINS AND MUCINS IN AMPULLARY CARCINOMA
ROLE OF INTEGRINS IN OVARIAN CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
VASCULAR ENDOTHELIAL GROWTH FACTOR C AND VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2 IN OVARIAN CANCER
USE OF MICROARRAY IN IMMUNOHISTOCHEMICAL LOCALIZATION OF SMAD IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF FAS IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF LEWIS Y ANTIGEN IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF ETS-1 FACTOR IN OVARIAN CARCINOMA
ROLE OF MCL-1 OVARIAN CARCINOMA
ROLE OF Elf-1 IN EPITHELIAL OVARIAN CARCINOMA: IMMUNOHISTOCHEMISTRY
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF OCT4 IN OVARIAN DYSGERMINOMA
IMMUNOHISTOCHEMICAL VALIDATION OF B7-H4 (DD-0110)
AS A BIOMARKER OF OVARIAN CANCER: CORRELATION WITH CA125
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF ALPHA
GLUTATHIONE S-TRANSFERASE IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF AMINOPEPTIDASES IN OVARIAN CARCINOMA
EXPRESSIONS OF ANGIOPOIETIN-1, ANGIOPOIETIN-2, AND
TIE2 IN NORMAL OVARY WITH CORPUS LUTEM AND IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF 1,25-
DIHYDROXYVITAMIN-D3-RECEPTORS IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF ANTIGENS IN NEUROENDOCRINE CARCINOMA OF THE OVARY AND ITS DIFFERENTIAL DIAGNOSTIC CONSIDERATIONS
ROLE OF IMMUNOHISTOCHEMISTRY IN ELUCIDATING LUNG CANCER METASTATIC TO THE OVARY FROM PRIMARY OVARIAN CARCINOMA
Classical histology has been augmented by
immunohistochemistry (the use of specific antibodies to stain particular molecular species in situ). Immunohistochemistry has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system.
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method.
- The only book available that translates molecular genetics into cancer diagnosis
- Methods were developed by internationally-recognized experts and presented in step-by-step manner
- Results of each Immunohistochemical and in situ hybridization are presented in the form of color illustrations
Review quote:
The basics of cell biology and molecular biology precede each section that describes defects in genetic/epigenetic control. Gaining an understanding of molecular genetic defects in cancer, authors then proceed to tell you how to measure the defects, which reinforces your understanding of the basic pathobiologic process. The latest therapies are discussed in the context of how to repair these molecular defects. Timely chapters on specific molecular topics in ovarian and gastrointestinal tumors are presented in the format of formal research papers. Extremely well written and succinct, a perfect marriage of information for students at any level, from medical student to professors of oncology.
-- David J. Dabbs, M.D., Chief of Pathology, Magee Womens Hospital University of Pittsburgh School of Medicine
Table of contents:
PART I. MOLECULAR GENETICS
IDENTIFICATION OF TUMOR-SPECIFIC GENES
THE POSTTRANSLATIONAL PHASE OF GENE EXPRESSION IN TUMOR DIAGNOSIS
ROLE OF BARD1 IN APOPTOSIS AND CANCER
ANGIOGENESIS, METASTASIS AND EPIGENETICS IN CANCER
CAN EFFECTOR CELLS REALLY EFFECT AN ANTITUMOR RESPONSE AS CANCER THERAPY?
CIRCULATING CANCER CELLS
CIRCULATING CANCER CELLS: FLOW CYTOMETRY, VIDEO-MICROSCOPY AND CONFOCAL LASER SCANNING MICROSCOPY
PART 2. GASTROINTESTINAL CARCINOMA
GASTROINTESTINAL CARCINOMA: AN INTRODUCTION ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF P53 IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF p53, Rb, AND p16 PROTEINS IN ANAL SQUAMOUS CELL CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF p53 AND VEGF IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF p150 IN
GASTRIC CARCINOMA: THE ASSOCIATION WITH P53, APOPTOSIS AND CELL PROLIFARATION
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF Ki-67 IN ADENOCARCINOMA OF LARGE INTESTINE
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF KIT/CD117 IN GASTROINTESTINAL STROMAL TUMORS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF BUB1 PROTEIN IN GASTRIC CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF EPIDERMAL GROWTH FACTOR IN GASTRIC TUMORS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF BETA-CATENIN AND MUCIN IN STOMACH CANCER
LOSS OF CYCLIN D2 EXPRESSION IN GASTRIC CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF E-CADHERIN DIFFUSE-TYPE GASTRIC CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF APC AND E-CADHERIN IN GASTRIC CARCINOMA
IMMUNOHISTOCHEMICAL EXPRESSION OF CHROMOGRANIN A (CgA) AND Leu-7 IN GASTROINTESTINAL CARCINOIDS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF MUC5B IN GASTRIC CARCINOMA
ANGIOGENIN IN GASTRIC CANCER AND ITS ROLES IN MALIGNANCY
ROLE OF HELICOBACTOR PYLORI IN GASTRIC CANCER
PROTEIN ALTERATIONS IN GASTRIC ADENOCARCINOMA
VESICLE PROTEINS IN NEUROENDOCRINE AND NONENDOCRINE TUMORS OF THE GASTROINTESTINAL TRACT
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF CASPASE-3 IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF PRL-3 PHOSPHATASE IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF DNA METHYLTRANSFERASE 1 PROTEIN IN GASTRIC CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
CYTOPLASMIC TREFOIL FACTOR FAMILY-2 IN GASTRIC CANCER
PART 3. OVARIAN CARCINOMA
OVARIAN CARCINOMA: AN ITNRODUCTION
METHODS FOR DETECTING GENETIC ABNORMALITIES IN OVARIAN CARCINOMA USING FLUORSCENCE IN SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY
QUANTITATIVE IMMUNOHISTOCHEMISTRY (Q-IHC) BY
DETERMINING THE NORM OF THE IMAGE DATA FILE
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF HER2/neu IN HIGH GRADE OVARIAN SEROUS PAPILLARY CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF BRCA1 IN OVARIAN CARCINOMA
ROLE OF BRCA/BRCA2 IN OVARIAN, FALLOPIAN TUBE AND PERITONEAL PAPILLARY SEROUS CARCINOMA
K-RAS MUTATIONS IN SEROUS BORDERLINE TUMORS OF THE OVARY
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF Ki-67 IN OVARIAN CARCINOMA
THE ROLE OF EXPRESSION OF ESTROGEN RECEPTOR BETA,
PROLIFERATING CELL NUCLEAR ANTIGEN AND P53 IN OVARIAN GRANULOSA CELL TUMORS
ROLE OF CYCLOOXYGENASE-2 EXPRESSION
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
CYCLOOXYGENASE-2 AND PEROXISOME PROLIFERATOR ACTIVATED-RECEPTOR IN EPITHELIAL OVARAIN TUMORS
CDX-2 IMMUNOSTAINING IN PRIMARY AND SECONDARY OVARIAN CARCINOMAS
EPITHELIAL OVARIAN CANCER AND THE E-CADHERIN-CATENIN COMPLEX
ROLE OF CYTOKERATIN IMMUNOHISTOCHEMISTRY IN THE DIFFERENTIAL DIAGNOSIS OF OVARIAN TUMORS
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
CYTOKERATINS AND MUCINS IN AMPULLARY CARCINOMA
ROLE OF INTEGRINS IN OVARIAN CANCER
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF
VASCULAR ENDOTHELIAL GROWTH FACTOR C AND VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 2 IN OVARIAN CANCER
USE OF MICROARRAY IN IMMUNOHISTOCHEMICAL LOCALIZATION OF SMAD IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF FAS IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF LEWIS Y ANTIGEN IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF ETS-1 FACTOR IN OVARIAN CARCINOMA
ROLE OF MCL-1 OVARIAN CARCINOMA
ROLE OF Elf-1 IN EPITHELIAL OVARIAN CARCINOMA: IMMUNOHISTOCHEMISTRY
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF OCT4 IN OVARIAN DYSGERMINOMA
IMMUNOHISTOCHEMICAL VALIDATION OF B7-H4 (DD-0110)
AS A BIOMARKER OF OVARIAN CANCER: CORRELATION WITH CA125
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF ALPHA
GLUTATHIONE S-TRANSFERASE IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF AMINOPEPTIDASES IN OVARIAN CARCINOMA
EXPRESSIONS OF ANGIOPOIETIN-1, ANGIOPOIETIN-2, AND
TIE2 IN NORMAL OVARY WITH CORPUS LUTEM AND IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF 1,25-
DIHYDROXYVITAMIN-D3-RECEPTORS IN OVARIAN CARCINOMA
ROLE OF IMMUNOHISTOCHEMICAL EXPRESSION OF ANTIGENS IN NEUROENDOCRINE CARCINOMA OF THE OVARY AND ITS DIFFERENTIAL DIAGNOSTIC CONSIDERATIONS
ROLE OF IMMUNOHISTOCHEMISTRY IN ELUCIDATING LUNG CANCER METASTATIC TO THE OVARY FROM PRIMARY OVARIAN CARCINOMA