Alf Hamann / Britta Engelhardt (eds.)

Handbook of Leukocyte Trafficking, w. CD-ROM

Mechanisms, Therapeutic Targets, and Methods
Herausgegeben:Hamann, Alf; Engelhardt, Britta

• Gebundenes Buch

Produktbeschreibung

This first handbook to comprehensively cover the field from molecular and cellular mechanisms to novel therapeutic strategies in the clinic comprises a selection of proven and time saving methods to study cell trafficking in vitro and in vivo. With movie data on CD-ROM.

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Written by known specialists in the field, this is the first comprehensive and timely overview of a central and expanding topic. Simultaneously an introduction and a description of the latest concepts, findings and methods, the handbook provides basic knowledge on technical issues required for those intending to research in the field. It covers the functional role of involved molecules and the cellular mechanisms, and addresses selected examples for their possible application in therapy - bridging the gap between trafficking mechanisms and novel therapeutic strategies. In addition, it includes selection of proven and timesaving methods for studying cell trafficking in vitro an in vivo.
The accompanying CD-ROM makes leukocyte migration videos available to a broad audience for the first time.

Produktdetails

• Verlag: Wiley-VCH
• Seitenzahl: 580
• Englisch
• Abmessung: 240mm
• Gewicht: 1204g
• ISBN-13: 9783527312283
• ISBN-10: 3527312285
• Artikelnr.: 13774556

Autorenporträt

Professor Alf Hamann is leader of the liaison group "Experimental Rheumatology" located at the German Arthritis Research Centre and the Charite hospital in Berlin. He established the research group in 1998 with a focus on basic and pathophysiological aspects of lymphocyte trafficking and immunoregulation.

Professor Britta Engelhardt started her career as research group leader at the Max-Planck Institute of Vascular Cell Biology in Münster, Germany, studying the molecular mechanisms of leukocyte migration into the central nervous system. Since 2003 she has been director of the renowned Theodor-Kocher-Institute at the University of Bern, Switzerland.

Inhaltsangabe

- Preface: A Mobile Society - The Constitutive Role of Cell Trafficking in the Organization of the Immune System (A. Hamann, B. Engelhardt)

MOLECULAR MECHANISMS
- The Multistep Model of Leukocyte Trafficking: a Personal Perspective from 15 Years later (E. C. Butcher)
- Capture and Rolling: Selectins and their Ligands (C. S. Bonder, P. Kubes)
- Chemokines and their Receptors: Biochemical, Structural and Biological Properties (M. Oppermann and R. Förster)
- Mechanisms of Leukocyte Integrin Activation (G. Constantin, C. Laudanna)
- Mechanisms of Leukocyte Transmigration: Role of Immunoglobulin Superfamily Molecules (F. M. Marelli-Berg, S. Nourshargh)
- The Endothelial Cell Basement Membrane and its Role in Leukocyte Extravasation (L. M. Sorokin)

TRAFFICKING IN VIVO
- Control of Homing Receptor Expression during Lymphocyte Differentiation, Activation and Function (D. J. Campbell)
- Trafficking of Lymphocyte Subpopulations (A. Hamann, C. N. Arnold, G. F. Debes)
- Trafficking of B Cells (R. A. Manz)
- Trafficking of Dendritic Cells (N. Romani, S. Holzmann, C. H. Tripp, M. Sixt, P. Stoitzner)

INFLAMMATION
- Molecular and Cellular Contributions to Selectin-dependent Leukocyte Adhesion under Flow (R. P. McEver)
- Mechanisms of Inflammation: Neutrophils (M. Sperandio, B. Walzog)
- Chemokines drive the Inflammatory Leukocyte Recruitment (S. Floess, A. Rot)
- Mechanism of Inflammation: Activation of the Endothelium (M. Clauss, C. E. Patterson)

TRAFFICKING MECHANISMS AS THERAPEUTIC TARGETS
- Integrins as Therapeutic Targets for Inflammatory Disease (M. Briskin)
- Chemokine Receptor Antagonists: From the Bench to the Clinic (S. Ribeiro, R. Horuk)

METHODS TO STUDY CELL TRAFFICKING
- Leukocyte/Endothelial Cell-Cell Interactions in vitro: Static Assays and Adhesion under Shear Stress (M. Hammel, O. Zilles, R. Hallmann, and S. Jennrich, K. Siemund, A. Hamann)
- Chemotaxis Assay: Analysis of Lymphocyte Subsets (K. Siegmund, G. F. Debes, A. Hamann)
- In vitro Transendothelial Migration Assay (R. Lyck, B. Engelhardt)
- Real Time in vitro Assays for Studying Leukocyte Transendothelial Migration under Physiological Flow Conditions (R. Alon, G. Cinamon, F. W. Luscinskas)
- Intravital Microscopy and in vitro Flow Chamber: Techniques to Study Leukocyte Adhesion under Flow and in Real Time (J. V. Stein)
- Immune Processes in the Light of Two-photon Microscopy (A. Flügel, N. Kawakami)
- Use of labeled Lymphocytes to Analyze Trafficking in vivo (K. Siegmund, A. Hamann)

Rezensionen

"Leukocyte Trafficking: Molecular Mechanisms, Therapeutic Targets, and Methods provides the fundamental knowledge on scientific and technical issues related to leukocyte trafficking. ... This volume covers many important aspects of the basic mechanisms, molecular pathways, cellular features, and possible therapeutic modulation of leukocyte trafficking. The volume also includes a CD-ROM, which provides leukocyte migration videos and all figures of the book in electronic form. In conclusion, this volume is an excellent source of information not only to students and academicians, but also to the researchers working in this area." - International Journal of Biological Macromolecules