W.E. Crusio / R.T. Gerlai (eds.)
Handbook of Molecular-Genetic Techniques for Brain and Behavior Research
Volume 13
Herausgeber: Crusio, Wim E; Gerlai, R T
W.E. Crusio / R.T. Gerlai (eds.)
Handbook of Molecular-Genetic Techniques for Brain and Behavior Research
Volume 13
Herausgeber: Crusio, Wim E; Gerlai, R T
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The book gives a broad overview of recombinant DNA techniques for the behavioral neuroscientist, with illustrative examples of applications. Species covered include rodents (mainly mice), Drosophila melanogaster, Caenorhabditis elegans and Danio rerio. Experimental techniques required to characterize the behavioral phenotypes of mutant animals is provided. Several aspects of novel molecular-genetic techniques are overviewed and possible research strategies are explained. The sections of the book start with general descriptions of techniques followed by illustrative examples. It is divided into…mehr
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The book gives a broad overview of recombinant DNA techniques for the behavioral neuroscientist, with illustrative examples of applications. Species covered include rodents (mainly mice), Drosophila melanogaster, Caenorhabditis elegans and Danio rerio. Experimental techniques required to characterize the behavioral phenotypes of mutant animals is provided. Several aspects of novel molecular-genetic techniques are overviewed and possible research strategies are explained. The sections of the book start with general descriptions of techniques followed by illustrative examples. It is divided into six sections. Section 1, bioinformatics and genomics research. Section 2, top-down strategies, where the researcher starts with the phenotype and then analyzes the associated genes; bottom-up strategies, where the physiological chain leading to a phenotype is analyzed starting from the gene product. Section 3, transgenic approaches in rodents including overexpressing foreign genes and gene-targeting; systemic manipulation approaches directly targeting the central nervous system and methods used with invertebrates. Section 4, methods used to evaluate relevant behavioral phenotypes, including learning and aggression. Section 5, examples on molecular brain research in man. Section 6, ethical aspects of research in this field.
Produktdetails
- Produktdetails
- Verlag: Elsevier Science
- Seitenzahl: 962
- Erscheinungstermin: 18. Oktober 1999
- Englisch
- Abmessung: 267mm x 199mm x 43mm
- Gewicht: 2458g
- ISBN-13: 9780444502391
- ISBN-10: 0444502394
- Artikelnr.: 20958713
- Verlag: Elsevier Science
- Seitenzahl: 962
- Erscheinungstermin: 18. Oktober 1999
- Englisch
- Abmessung: 267mm x 199mm x 43mm
- Gewicht: 2458g
- ISBN-13: 9780444502391
- ISBN-10: 0444502394
- Artikelnr.: 20958713
Dedication. Preface. Behavioral neurogenetics: the new synthesis (W.E.
Crusio, R.T. Gerlai). Acknowledgements. List of Contributors. 1.
Neurobehavioral Informatics and Statistics. 1.1. The multi-dimensional
database requirements of brain information in the era of rapid gene
identification (F.E. Bloom, W.G. Young). 1.2. Bioinformatics and
neuroscience in the post-genomic era (B.W.S. Sobral, M.M. Harpold). 1.3.
PsycheMap - an online database of psychiatric genetics linkage,
association, and genome mapping projects (N.M. Williams, I. Fenton, M.J.
Owen). 1.4. Experimental design and statistical inference (D. Wahlsten).
2. Searching for New Genes. 2.1. Spontaneous and Induced Mutations with
Effects on Neural and Behavioral Traits. 2.1.1. Mapping single locus
mutations in mice: towards gene identification of neurological traits (W.N.
Frankel, B.A. Taylor). 2.1.2. Experimental strategies for mapping
quantitative trait loci (QTL) analysis in laboratory animals (D.A. Blizard,
A. Darvasi). 2.1.3. Linkage strategies for mapping genes for complex traits
in man (L. Almasy, J. Blangero). 2.1.4. Genetic association studies in
behavioral neuroscience (P. Gorwood). 2.1.5. DNA pooling in allelic
association studies (N.M. Williams, M.J. Owen). 2.1.6. Research strategies
for the analysis of neurological mutants of the mouse (C. Sotelo, J.
Mariani). 2.1.7. Genetic dissection of mouse behavior using induced
mutagenesis (L.H. Pinto, J.S. Takahashi). 2.1.8. Mutagenesis in zebra fish:
studying the brain dopamine systems (S. Guo, W. Driever, A. Rosenthal).
2.1.9. Behavioral and electrophysiological screens for isolating zebra fish
mutants with visual system defects (J.E. Dowling). 2.2. Finding Genes with
Phenotypical Effects on Neural and Behavioral Phenotypes on the Basis of
Gene-Expression. 2.2.1. Subtractive cDNA hybridization and the brain: then,
now and tomorrow (J.B. Watson). 2.2.2. Applying differential display to
brain research (C.V. Mello, E.D. Jarvis). 2.2.3. Brain region-specific
genes: the hippocampus (B.S. Pickard, B.J. Davies, K.A. Rose, G. Stapleton,
M. Steel, R. Lathe). 2.2.4. Application of real-time RT-PCR for
quantification of gene expression (J. Winer, N. Shinsky, R. Gerlai, P.M.
Williams). 2.2.5. Analyzing genomic DNA discordance between monozygotic
twins (J. Bouchard, C. Foulon, N. Storm, G.H. Nguyen, C.L. Smith). 3.
Manipulating Known Genes. 3.1. Transgenic Approaches in Rodents. 3.1.1.
Embryonic stem cells and gene targeting (A. Wynshaw-Boris, L. Garrett, A.
Chen, C. Barlow). 3.1.2. Generation of transgenic mice by pronuclear DNA
injection (A. Wynshaw-Boris, L. Garrett, A. Chen, C. Barlow). 3.1.3. Brain
region-specific and temporally restricted gene knockout using the Cre
recombinase system (J.Z. Tsien). 3.1.4. Regulated temporal and spatial
expression of mutants of CaMKII and calcineurin with the
tetracycline-controlled transactivator (tTA) and reverse tTA (rtTA) systems
(I.M. Mansuy, M. Mayford, E.R. Kandel). 3.1.5. The use of targeted point
mutants in the study of learning and memory (K.P. Giese). 3.1.6. Genetic
dissection of a postsynaptic multiprotein complex controlling synaptic
plasticity and learning in the mouse (S.G.N. Grant). 3.1.7. Molecular
genetic analysis of glutamate receptor function in long-term potentiation
in the mouse hippocampus (Z. Jia, Y.M. Lu, N. Agopyan, J. Roder). 3.1.8.
Targeting aggression in mice (R.J. Nelson, L.J. Kriegsfeld). 3.1.9.
Behavioral analysis of Dvl1-deficient mice reveals a role for the Dvl1 gene
in social behaviors and sensorimotor gating (R. Paylor, N. Lijam, M.P.
McDonald, J.N. Crawley, D.J. Sussman, A. Wynshaw-Boris). 3.1.10. Targeting
genes associated with mammalian behavior: past mistakes and future
solutions (R. Gerlai). 3.2. Systemic Manipulation. 3.2.1. Gene transfer and
therapy in the CNS (M.-C. Senut, S.T. Suhr, F.H. Gage). 3.2.2. Adenovirus
vectors for gene transfer into the central nervous system (M. Barkats, O.
Corti, J. Mallet). 3.2.3. Regulatable adenoviral technology in behavioural
neuroscience (J.B. Uney, B. Geddes, E.C. Warburton, T. Harding). 3.2.4.
Antisense oligonucleotides to selectively suppress gene expression in the
brain (G. Pollio, A. Maggi). 3.2.5. Application of recombinant proteins,
peptides and antibodies in exploring the role of Src in regulating synaptic
function (M.W. Salter). 3.2.6. The use of immunoadhesins in neurobiology
(D.L. Shelton). 3.2.7. Protein targeting in the functional analysis of EphA
receptors: the use of immunoadhesins (R. Gerlai, N. Shinsky, A. Shih, P.
Williams, J. Winer, M. Armanini, P. Moran, B. Cairns, J. Winslow, W.-Q.
Gao, H.S. Phillips). 3.3. Invertebrates. 3.3.1. A novel approach to
Drosophila neurophysiology: the targeted expression of aequorin (P. Rosay,
K. Kaiser, J.D. Armstrong). 3.3.2. Behavior-genetic and molecular analysis
of naturally occurring variation in Drosophila larval foraging behavior
(M.B. Sokolowski, C.A.L. Riedl). 3.3.3. Structure-function analysis of the
Drosophila optic lobes (G. Pflugfelder). 3.3.4. Testing associative
learning in Drosophila (T. Préat). 3.3.5. Caenorhabditis elegans and the
genetics of learning (K.R. Peters, J.A. Galloway, C.H. Rankin). 3.3.6.
Forward genetic approaches in the analysis of Caenorhabditis elegans (A.C.
Hart). 3.3.7. Analyzing neuropeptide function in Caenorhabditis elegans by
reverse genetics (C. Li). 4. Evaluating Behavioral Phenotypes in Rodents.
4.1. Ethological approaches in behavioral neurogenetic research (R.
Gerlai). 4.2. What animals remember about past events: an ethological
approach (N.S. Clayton). 4.3. Motor performance of spontaneous murine
mutations with cerebellar atrophy (R. Lalonde, C. Strazielle). 4.4.
Methodological considerations for testing learning in mice (W.E. Crusio).
4.5. Drug and alcohol dependence-related behaviors (J.C. Crabbe, C.L.
Cunningham). 4.6. Evaluating anxiety in rodents (J.N. Crawley). 4.7. A
neurobehavioral system approach in rats to study the molecular biology of
fear (J.B. Rosen, S. Malkani, K. Wallace, B. Thompson). 4.8. Measuring
aggression in the mouse (P.L. Roubertoux, I. Le Roy, S. Mortaud, F.
Perez-Diaz, S. Tordjman). 4.9. Methodological issues in the assessment of
behavioral development in laboratory mice (P.E. Wainwright). 4.10.
Understanding maternal behavior: analyses of behavior, c-Fos expression and
calmodulin binding proteins in the medial preoptic area and other areas of
the rat brain (A.S. Fleming, D.H. O'Day). 4.11. Measuring rodent
exploratory behavior (C. Belzung). 5. Human Neurobehavioral Disorders: from
Molecular Genetics to Genetic Animal Modes. 5.1. Psychiatric genetics - a
current perspective (D.F. Levinson). 5.2. Defining phenotypes for
psychiatric genetics (M. Nosten-Bertrand, F. Bellivier, M. Leboyer). 5.3.
Trinucleotide repeat disorders (G. Sandberg, K. Lindblad, B.A. Oostra, M.
Schalling). 5.4. Finding liability genes for schizophrenia (N.J.O.
Jacobsen, N.M. Williams, M.J. Owen). 5.5. Genetics of idiopathic epilepsy
(L. Bate, M. Gardiner). 5.6. Identification and functional analysis of
genes and genetic risk factors in Alzheimer's disease (C. De Jonghe, C. Van
Broeckhoven). 5.7. Aging, Alzheimer's disease and frameshift mutations
(W.H. Van den Hurk, F.W. Van Leeuwen, G.J.M. Martens). 5.8. Transgenic
mouse models of Alzheimer's disease (K. Duff). 5.9. Modelling Down syndrome
in mice (M. Dierssen, M. Pritchard, C. Fillat, M. Arbonés, J.M. Aran, J.
Flórez, X. Estivill). 6. Ethical Considerations. 6.1. Genes and human
behavior: scientific and ethical implications of the human genome project
(J. Beckwith). 6.2. Ethical issues and psychiatric genetics (A.E. Farmer,
P. McGuffin). 6.3. Ethical implications of knock-out and transgenesis
techniques for animal research (L.M. Houdebine). Subject index.
Crusio, R.T. Gerlai). Acknowledgements. List of Contributors. 1.
Neurobehavioral Informatics and Statistics. 1.1. The multi-dimensional
database requirements of brain information in the era of rapid gene
identification (F.E. Bloom, W.G. Young). 1.2. Bioinformatics and
neuroscience in the post-genomic era (B.W.S. Sobral, M.M. Harpold). 1.3.
PsycheMap - an online database of psychiatric genetics linkage,
association, and genome mapping projects (N.M. Williams, I. Fenton, M.J.
Owen). 1.4. Experimental design and statistical inference (D. Wahlsten).
2. Searching for New Genes. 2.1. Spontaneous and Induced Mutations with
Effects on Neural and Behavioral Traits. 2.1.1. Mapping single locus
mutations in mice: towards gene identification of neurological traits (W.N.
Frankel, B.A. Taylor). 2.1.2. Experimental strategies for mapping
quantitative trait loci (QTL) analysis in laboratory animals (D.A. Blizard,
A. Darvasi). 2.1.3. Linkage strategies for mapping genes for complex traits
in man (L. Almasy, J. Blangero). 2.1.4. Genetic association studies in
behavioral neuroscience (P. Gorwood). 2.1.5. DNA pooling in allelic
association studies (N.M. Williams, M.J. Owen). 2.1.6. Research strategies
for the analysis of neurological mutants of the mouse (C. Sotelo, J.
Mariani). 2.1.7. Genetic dissection of mouse behavior using induced
mutagenesis (L.H. Pinto, J.S. Takahashi). 2.1.8. Mutagenesis in zebra fish:
studying the brain dopamine systems (S. Guo, W. Driever, A. Rosenthal).
2.1.9. Behavioral and electrophysiological screens for isolating zebra fish
mutants with visual system defects (J.E. Dowling). 2.2. Finding Genes with
Phenotypical Effects on Neural and Behavioral Phenotypes on the Basis of
Gene-Expression. 2.2.1. Subtractive cDNA hybridization and the brain: then,
now and tomorrow (J.B. Watson). 2.2.2. Applying differential display to
brain research (C.V. Mello, E.D. Jarvis). 2.2.3. Brain region-specific
genes: the hippocampus (B.S. Pickard, B.J. Davies, K.A. Rose, G. Stapleton,
M. Steel, R. Lathe). 2.2.4. Application of real-time RT-PCR for
quantification of gene expression (J. Winer, N. Shinsky, R. Gerlai, P.M.
Williams). 2.2.5. Analyzing genomic DNA discordance between monozygotic
twins (J. Bouchard, C. Foulon, N. Storm, G.H. Nguyen, C.L. Smith). 3.
Manipulating Known Genes. 3.1. Transgenic Approaches in Rodents. 3.1.1.
Embryonic stem cells and gene targeting (A. Wynshaw-Boris, L. Garrett, A.
Chen, C. Barlow). 3.1.2. Generation of transgenic mice by pronuclear DNA
injection (A. Wynshaw-Boris, L. Garrett, A. Chen, C. Barlow). 3.1.3. Brain
region-specific and temporally restricted gene knockout using the Cre
recombinase system (J.Z. Tsien). 3.1.4. Regulated temporal and spatial
expression of mutants of CaMKII and calcineurin with the
tetracycline-controlled transactivator (tTA) and reverse tTA (rtTA) systems
(I.M. Mansuy, M. Mayford, E.R. Kandel). 3.1.5. The use of targeted point
mutants in the study of learning and memory (K.P. Giese). 3.1.6. Genetic
dissection of a postsynaptic multiprotein complex controlling synaptic
plasticity and learning in the mouse (S.G.N. Grant). 3.1.7. Molecular
genetic analysis of glutamate receptor function in long-term potentiation
in the mouse hippocampus (Z. Jia, Y.M. Lu, N. Agopyan, J. Roder). 3.1.8.
Targeting aggression in mice (R.J. Nelson, L.J. Kriegsfeld). 3.1.9.
Behavioral analysis of Dvl1-deficient mice reveals a role for the Dvl1 gene
in social behaviors and sensorimotor gating (R. Paylor, N. Lijam, M.P.
McDonald, J.N. Crawley, D.J. Sussman, A. Wynshaw-Boris). 3.1.10. Targeting
genes associated with mammalian behavior: past mistakes and future
solutions (R. Gerlai). 3.2. Systemic Manipulation. 3.2.1. Gene transfer and
therapy in the CNS (M.-C. Senut, S.T. Suhr, F.H. Gage). 3.2.2. Adenovirus
vectors for gene transfer into the central nervous system (M. Barkats, O.
Corti, J. Mallet). 3.2.3. Regulatable adenoviral technology in behavioural
neuroscience (J.B. Uney, B. Geddes, E.C. Warburton, T. Harding). 3.2.4.
Antisense oligonucleotides to selectively suppress gene expression in the
brain (G. Pollio, A. Maggi). 3.2.5. Application of recombinant proteins,
peptides and antibodies in exploring the role of Src in regulating synaptic
function (M.W. Salter). 3.2.6. The use of immunoadhesins in neurobiology
(D.L. Shelton). 3.2.7. Protein targeting in the functional analysis of EphA
receptors: the use of immunoadhesins (R. Gerlai, N. Shinsky, A. Shih, P.
Williams, J. Winer, M. Armanini, P. Moran, B. Cairns, J. Winslow, W.-Q.
Gao, H.S. Phillips). 3.3. Invertebrates. 3.3.1. A novel approach to
Drosophila neurophysiology: the targeted expression of aequorin (P. Rosay,
K. Kaiser, J.D. Armstrong). 3.3.2. Behavior-genetic and molecular analysis
of naturally occurring variation in Drosophila larval foraging behavior
(M.B. Sokolowski, C.A.L. Riedl). 3.3.3. Structure-function analysis of the
Drosophila optic lobes (G. Pflugfelder). 3.3.4. Testing associative
learning in Drosophila (T. Préat). 3.3.5. Caenorhabditis elegans and the
genetics of learning (K.R. Peters, J.A. Galloway, C.H. Rankin). 3.3.6.
Forward genetic approaches in the analysis of Caenorhabditis elegans (A.C.
Hart). 3.3.7. Analyzing neuropeptide function in Caenorhabditis elegans by
reverse genetics (C. Li). 4. Evaluating Behavioral Phenotypes in Rodents.
4.1. Ethological approaches in behavioral neurogenetic research (R.
Gerlai). 4.2. What animals remember about past events: an ethological
approach (N.S. Clayton). 4.3. Motor performance of spontaneous murine
mutations with cerebellar atrophy (R. Lalonde, C. Strazielle). 4.4.
Methodological considerations for testing learning in mice (W.E. Crusio).
4.5. Drug and alcohol dependence-related behaviors (J.C. Crabbe, C.L.
Cunningham). 4.6. Evaluating anxiety in rodents (J.N. Crawley). 4.7. A
neurobehavioral system approach in rats to study the molecular biology of
fear (J.B. Rosen, S. Malkani, K. Wallace, B. Thompson). 4.8. Measuring
aggression in the mouse (P.L. Roubertoux, I. Le Roy, S. Mortaud, F.
Perez-Diaz, S. Tordjman). 4.9. Methodological issues in the assessment of
behavioral development in laboratory mice (P.E. Wainwright). 4.10.
Understanding maternal behavior: analyses of behavior, c-Fos expression and
calmodulin binding proteins in the medial preoptic area and other areas of
the rat brain (A.S. Fleming, D.H. O'Day). 4.11. Measuring rodent
exploratory behavior (C. Belzung). 5. Human Neurobehavioral Disorders: from
Molecular Genetics to Genetic Animal Modes. 5.1. Psychiatric genetics - a
current perspective (D.F. Levinson). 5.2. Defining phenotypes for
psychiatric genetics (M. Nosten-Bertrand, F. Bellivier, M. Leboyer). 5.3.
Trinucleotide repeat disorders (G. Sandberg, K. Lindblad, B.A. Oostra, M.
Schalling). 5.4. Finding liability genes for schizophrenia (N.J.O.
Jacobsen, N.M. Williams, M.J. Owen). 5.5. Genetics of idiopathic epilepsy
(L. Bate, M. Gardiner). 5.6. Identification and functional analysis of
genes and genetic risk factors in Alzheimer's disease (C. De Jonghe, C. Van
Broeckhoven). 5.7. Aging, Alzheimer's disease and frameshift mutations
(W.H. Van den Hurk, F.W. Van Leeuwen, G.J.M. Martens). 5.8. Transgenic
mouse models of Alzheimer's disease (K. Duff). 5.9. Modelling Down syndrome
in mice (M. Dierssen, M. Pritchard, C. Fillat, M. Arbonés, J.M. Aran, J.
Flórez, X. Estivill). 6. Ethical Considerations. 6.1. Genes and human
behavior: scientific and ethical implications of the human genome project
(J. Beckwith). 6.2. Ethical issues and psychiatric genetics (A.E. Farmer,
P. McGuffin). 6.3. Ethical implications of knock-out and transgenesis
techniques for animal research (L.M. Houdebine). Subject index.
Dedication. Preface. Behavioral neurogenetics: the new synthesis (W.E.
Crusio, R.T. Gerlai). Acknowledgements. List of Contributors. 1.
Neurobehavioral Informatics and Statistics. 1.1. The multi-dimensional
database requirements of brain information in the era of rapid gene
identification (F.E. Bloom, W.G. Young). 1.2. Bioinformatics and
neuroscience in the post-genomic era (B.W.S. Sobral, M.M. Harpold). 1.3.
PsycheMap - an online database of psychiatric genetics linkage,
association, and genome mapping projects (N.M. Williams, I. Fenton, M.J.
Owen). 1.4. Experimental design and statistical inference (D. Wahlsten).
2. Searching for New Genes. 2.1. Spontaneous and Induced Mutations with
Effects on Neural and Behavioral Traits. 2.1.1. Mapping single locus
mutations in mice: towards gene identification of neurological traits (W.N.
Frankel, B.A. Taylor). 2.1.2. Experimental strategies for mapping
quantitative trait loci (QTL) analysis in laboratory animals (D.A. Blizard,
A. Darvasi). 2.1.3. Linkage strategies for mapping genes for complex traits
in man (L. Almasy, J. Blangero). 2.1.4. Genetic association studies in
behavioral neuroscience (P. Gorwood). 2.1.5. DNA pooling in allelic
association studies (N.M. Williams, M.J. Owen). 2.1.6. Research strategies
for the analysis of neurological mutants of the mouse (C. Sotelo, J.
Mariani). 2.1.7. Genetic dissection of mouse behavior using induced
mutagenesis (L.H. Pinto, J.S. Takahashi). 2.1.8. Mutagenesis in zebra fish:
studying the brain dopamine systems (S. Guo, W. Driever, A. Rosenthal).
2.1.9. Behavioral and electrophysiological screens for isolating zebra fish
mutants with visual system defects (J.E. Dowling). 2.2. Finding Genes with
Phenotypical Effects on Neural and Behavioral Phenotypes on the Basis of
Gene-Expression. 2.2.1. Subtractive cDNA hybridization and the brain: then,
now and tomorrow (J.B. Watson). 2.2.2. Applying differential display to
brain research (C.V. Mello, E.D. Jarvis). 2.2.3. Brain region-specific
genes: the hippocampus (B.S. Pickard, B.J. Davies, K.A. Rose, G. Stapleton,
M. Steel, R. Lathe). 2.2.4. Application of real-time RT-PCR for
quantification of gene expression (J. Winer, N. Shinsky, R. Gerlai, P.M.
Williams). 2.2.5. Analyzing genomic DNA discordance between monozygotic
twins (J. Bouchard, C. Foulon, N. Storm, G.H. Nguyen, C.L. Smith). 3.
Manipulating Known Genes. 3.1. Transgenic Approaches in Rodents. 3.1.1.
Embryonic stem cells and gene targeting (A. Wynshaw-Boris, L. Garrett, A.
Chen, C. Barlow). 3.1.2. Generation of transgenic mice by pronuclear DNA
injection (A. Wynshaw-Boris, L. Garrett, A. Chen, C. Barlow). 3.1.3. Brain
region-specific and temporally restricted gene knockout using the Cre
recombinase system (J.Z. Tsien). 3.1.4. Regulated temporal and spatial
expression of mutants of CaMKII and calcineurin with the
tetracycline-controlled transactivator (tTA) and reverse tTA (rtTA) systems
(I.M. Mansuy, M. Mayford, E.R. Kandel). 3.1.5. The use of targeted point
mutants in the study of learning and memory (K.P. Giese). 3.1.6. Genetic
dissection of a postsynaptic multiprotein complex controlling synaptic
plasticity and learning in the mouse (S.G.N. Grant). 3.1.7. Molecular
genetic analysis of glutamate receptor function in long-term potentiation
in the mouse hippocampus (Z. Jia, Y.M. Lu, N. Agopyan, J. Roder). 3.1.8.
Targeting aggression in mice (R.J. Nelson, L.J. Kriegsfeld). 3.1.9.
Behavioral analysis of Dvl1-deficient mice reveals a role for the Dvl1 gene
in social behaviors and sensorimotor gating (R. Paylor, N. Lijam, M.P.
McDonald, J.N. Crawley, D.J. Sussman, A. Wynshaw-Boris). 3.1.10. Targeting
genes associated with mammalian behavior: past mistakes and future
solutions (R. Gerlai). 3.2. Systemic Manipulation. 3.2.1. Gene transfer and
therapy in the CNS (M.-C. Senut, S.T. Suhr, F.H. Gage). 3.2.2. Adenovirus
vectors for gene transfer into the central nervous system (M. Barkats, O.
Corti, J. Mallet). 3.2.3. Regulatable adenoviral technology in behavioural
neuroscience (J.B. Uney, B. Geddes, E.C. Warburton, T. Harding). 3.2.4.
Antisense oligonucleotides to selectively suppress gene expression in the
brain (G. Pollio, A. Maggi). 3.2.5. Application of recombinant proteins,
peptides and antibodies in exploring the role of Src in regulating synaptic
function (M.W. Salter). 3.2.6. The use of immunoadhesins in neurobiology
(D.L. Shelton). 3.2.7. Protein targeting in the functional analysis of EphA
receptors: the use of immunoadhesins (R. Gerlai, N. Shinsky, A. Shih, P.
Williams, J. Winer, M. Armanini, P. Moran, B. Cairns, J. Winslow, W.-Q.
Gao, H.S. Phillips). 3.3. Invertebrates. 3.3.1. A novel approach to
Drosophila neurophysiology: the targeted expression of aequorin (P. Rosay,
K. Kaiser, J.D. Armstrong). 3.3.2. Behavior-genetic and molecular analysis
of naturally occurring variation in Drosophila larval foraging behavior
(M.B. Sokolowski, C.A.L. Riedl). 3.3.3. Structure-function analysis of the
Drosophila optic lobes (G. Pflugfelder). 3.3.4. Testing associative
learning in Drosophila (T. Préat). 3.3.5. Caenorhabditis elegans and the
genetics of learning (K.R. Peters, J.A. Galloway, C.H. Rankin). 3.3.6.
Forward genetic approaches in the analysis of Caenorhabditis elegans (A.C.
Hart). 3.3.7. Analyzing neuropeptide function in Caenorhabditis elegans by
reverse genetics (C. Li). 4. Evaluating Behavioral Phenotypes in Rodents.
4.1. Ethological approaches in behavioral neurogenetic research (R.
Gerlai). 4.2. What animals remember about past events: an ethological
approach (N.S. Clayton). 4.3. Motor performance of spontaneous murine
mutations with cerebellar atrophy (R. Lalonde, C. Strazielle). 4.4.
Methodological considerations for testing learning in mice (W.E. Crusio).
4.5. Drug and alcohol dependence-related behaviors (J.C. Crabbe, C.L.
Cunningham). 4.6. Evaluating anxiety in rodents (J.N. Crawley). 4.7. A
neurobehavioral system approach in rats to study the molecular biology of
fear (J.B. Rosen, S. Malkani, K. Wallace, B. Thompson). 4.8. Measuring
aggression in the mouse (P.L. Roubertoux, I. Le Roy, S. Mortaud, F.
Perez-Diaz, S. Tordjman). 4.9. Methodological issues in the assessment of
behavioral development in laboratory mice (P.E. Wainwright). 4.10.
Understanding maternal behavior: analyses of behavior, c-Fos expression and
calmodulin binding proteins in the medial preoptic area and other areas of
the rat brain (A.S. Fleming, D.H. O'Day). 4.11. Measuring rodent
exploratory behavior (C. Belzung). 5. Human Neurobehavioral Disorders: from
Molecular Genetics to Genetic Animal Modes. 5.1. Psychiatric genetics - a
current perspective (D.F. Levinson). 5.2. Defining phenotypes for
psychiatric genetics (M. Nosten-Bertrand, F. Bellivier, M. Leboyer). 5.3.
Trinucleotide repeat disorders (G. Sandberg, K. Lindblad, B.A. Oostra, M.
Schalling). 5.4. Finding liability genes for schizophrenia (N.J.O.
Jacobsen, N.M. Williams, M.J. Owen). 5.5. Genetics of idiopathic epilepsy
(L. Bate, M. Gardiner). 5.6. Identification and functional analysis of
genes and genetic risk factors in Alzheimer's disease (C. De Jonghe, C. Van
Broeckhoven). 5.7. Aging, Alzheimer's disease and frameshift mutations
(W.H. Van den Hurk, F.W. Van Leeuwen, G.J.M. Martens). 5.8. Transgenic
mouse models of Alzheimer's disease (K. Duff). 5.9. Modelling Down syndrome
in mice (M. Dierssen, M. Pritchard, C. Fillat, M. Arbonés, J.M. Aran, J.
Flórez, X. Estivill). 6. Ethical Considerations. 6.1. Genes and human
behavior: scientific and ethical implications of the human genome project
(J. Beckwith). 6.2. Ethical issues and psychiatric genetics (A.E. Farmer,
P. McGuffin). 6.3. Ethical implications of knock-out and transgenesis
techniques for animal research (L.M. Houdebine). Subject index.
Crusio, R.T. Gerlai). Acknowledgements. List of Contributors. 1.
Neurobehavioral Informatics and Statistics. 1.1. The multi-dimensional
database requirements of brain information in the era of rapid gene
identification (F.E. Bloom, W.G. Young). 1.2. Bioinformatics and
neuroscience in the post-genomic era (B.W.S. Sobral, M.M. Harpold). 1.3.
PsycheMap - an online database of psychiatric genetics linkage,
association, and genome mapping projects (N.M. Williams, I. Fenton, M.J.
Owen). 1.4. Experimental design and statistical inference (D. Wahlsten).
2. Searching for New Genes. 2.1. Spontaneous and Induced Mutations with
Effects on Neural and Behavioral Traits. 2.1.1. Mapping single locus
mutations in mice: towards gene identification of neurological traits (W.N.
Frankel, B.A. Taylor). 2.1.2. Experimental strategies for mapping
quantitative trait loci (QTL) analysis in laboratory animals (D.A. Blizard,
A. Darvasi). 2.1.3. Linkage strategies for mapping genes for complex traits
in man (L. Almasy, J. Blangero). 2.1.4. Genetic association studies in
behavioral neuroscience (P. Gorwood). 2.1.5. DNA pooling in allelic
association studies (N.M. Williams, M.J. Owen). 2.1.6. Research strategies
for the analysis of neurological mutants of the mouse (C. Sotelo, J.
Mariani). 2.1.7. Genetic dissection of mouse behavior using induced
mutagenesis (L.H. Pinto, J.S. Takahashi). 2.1.8. Mutagenesis in zebra fish:
studying the brain dopamine systems (S. Guo, W. Driever, A. Rosenthal).
2.1.9. Behavioral and electrophysiological screens for isolating zebra fish
mutants with visual system defects (J.E. Dowling). 2.2. Finding Genes with
Phenotypical Effects on Neural and Behavioral Phenotypes on the Basis of
Gene-Expression. 2.2.1. Subtractive cDNA hybridization and the brain: then,
now and tomorrow (J.B. Watson). 2.2.2. Applying differential display to
brain research (C.V. Mello, E.D. Jarvis). 2.2.3. Brain region-specific
genes: the hippocampus (B.S. Pickard, B.J. Davies, K.A. Rose, G. Stapleton,
M. Steel, R. Lathe). 2.2.4. Application of real-time RT-PCR for
quantification of gene expression (J. Winer, N. Shinsky, R. Gerlai, P.M.
Williams). 2.2.5. Analyzing genomic DNA discordance between monozygotic
twins (J. Bouchard, C. Foulon, N. Storm, G.H. Nguyen, C.L. Smith). 3.
Manipulating Known Genes. 3.1. Transgenic Approaches in Rodents. 3.1.1.
Embryonic stem cells and gene targeting (A. Wynshaw-Boris, L. Garrett, A.
Chen, C. Barlow). 3.1.2. Generation of transgenic mice by pronuclear DNA
injection (A. Wynshaw-Boris, L. Garrett, A. Chen, C. Barlow). 3.1.3. Brain
region-specific and temporally restricted gene knockout using the Cre
recombinase system (J.Z. Tsien). 3.1.4. Regulated temporal and spatial
expression of mutants of CaMKII and calcineurin with the
tetracycline-controlled transactivator (tTA) and reverse tTA (rtTA) systems
(I.M. Mansuy, M. Mayford, E.R. Kandel). 3.1.5. The use of targeted point
mutants in the study of learning and memory (K.P. Giese). 3.1.6. Genetic
dissection of a postsynaptic multiprotein complex controlling synaptic
plasticity and learning in the mouse (S.G.N. Grant). 3.1.7. Molecular
genetic analysis of glutamate receptor function in long-term potentiation
in the mouse hippocampus (Z. Jia, Y.M. Lu, N. Agopyan, J. Roder). 3.1.8.
Targeting aggression in mice (R.J. Nelson, L.J. Kriegsfeld). 3.1.9.
Behavioral analysis of Dvl1-deficient mice reveals a role for the Dvl1 gene
in social behaviors and sensorimotor gating (R. Paylor, N. Lijam, M.P.
McDonald, J.N. Crawley, D.J. Sussman, A. Wynshaw-Boris). 3.1.10. Targeting
genes associated with mammalian behavior: past mistakes and future
solutions (R. Gerlai). 3.2. Systemic Manipulation. 3.2.1. Gene transfer and
therapy in the CNS (M.-C. Senut, S.T. Suhr, F.H. Gage). 3.2.2. Adenovirus
vectors for gene transfer into the central nervous system (M. Barkats, O.
Corti, J. Mallet). 3.2.3. Regulatable adenoviral technology in behavioural
neuroscience (J.B. Uney, B. Geddes, E.C. Warburton, T. Harding). 3.2.4.
Antisense oligonucleotides to selectively suppress gene expression in the
brain (G. Pollio, A. Maggi). 3.2.5. Application of recombinant proteins,
peptides and antibodies in exploring the role of Src in regulating synaptic
function (M.W. Salter). 3.2.6. The use of immunoadhesins in neurobiology
(D.L. Shelton). 3.2.7. Protein targeting in the functional analysis of EphA
receptors: the use of immunoadhesins (R. Gerlai, N. Shinsky, A. Shih, P.
Williams, J. Winer, M. Armanini, P. Moran, B. Cairns, J. Winslow, W.-Q.
Gao, H.S. Phillips). 3.3. Invertebrates. 3.3.1. A novel approach to
Drosophila neurophysiology: the targeted expression of aequorin (P. Rosay,
K. Kaiser, J.D. Armstrong). 3.3.2. Behavior-genetic and molecular analysis
of naturally occurring variation in Drosophila larval foraging behavior
(M.B. Sokolowski, C.A.L. Riedl). 3.3.3. Structure-function analysis of the
Drosophila optic lobes (G. Pflugfelder). 3.3.4. Testing associative
learning in Drosophila (T. Préat). 3.3.5. Caenorhabditis elegans and the
genetics of learning (K.R. Peters, J.A. Galloway, C.H. Rankin). 3.3.6.
Forward genetic approaches in the analysis of Caenorhabditis elegans (A.C.
Hart). 3.3.7. Analyzing neuropeptide function in Caenorhabditis elegans by
reverse genetics (C. Li). 4. Evaluating Behavioral Phenotypes in Rodents.
4.1. Ethological approaches in behavioral neurogenetic research (R.
Gerlai). 4.2. What animals remember about past events: an ethological
approach (N.S. Clayton). 4.3. Motor performance of spontaneous murine
mutations with cerebellar atrophy (R. Lalonde, C. Strazielle). 4.4.
Methodological considerations for testing learning in mice (W.E. Crusio).
4.5. Drug and alcohol dependence-related behaviors (J.C. Crabbe, C.L.
Cunningham). 4.6. Evaluating anxiety in rodents (J.N. Crawley). 4.7. A
neurobehavioral system approach in rats to study the molecular biology of
fear (J.B. Rosen, S. Malkani, K. Wallace, B. Thompson). 4.8. Measuring
aggression in the mouse (P.L. Roubertoux, I. Le Roy, S. Mortaud, F.
Perez-Diaz, S. Tordjman). 4.9. Methodological issues in the assessment of
behavioral development in laboratory mice (P.E. Wainwright). 4.10.
Understanding maternal behavior: analyses of behavior, c-Fos expression and
calmodulin binding proteins in the medial preoptic area and other areas of
the rat brain (A.S. Fleming, D.H. O'Day). 4.11. Measuring rodent
exploratory behavior (C. Belzung). 5. Human Neurobehavioral Disorders: from
Molecular Genetics to Genetic Animal Modes. 5.1. Psychiatric genetics - a
current perspective (D.F. Levinson). 5.2. Defining phenotypes for
psychiatric genetics (M. Nosten-Bertrand, F. Bellivier, M. Leboyer). 5.3.
Trinucleotide repeat disorders (G. Sandberg, K. Lindblad, B.A. Oostra, M.
Schalling). 5.4. Finding liability genes for schizophrenia (N.J.O.
Jacobsen, N.M. Williams, M.J. Owen). 5.5. Genetics of idiopathic epilepsy
(L. Bate, M. Gardiner). 5.6. Identification and functional analysis of
genes and genetic risk factors in Alzheimer's disease (C. De Jonghe, C. Van
Broeckhoven). 5.7. Aging, Alzheimer's disease and frameshift mutations
(W.H. Van den Hurk, F.W. Van Leeuwen, G.J.M. Martens). 5.8. Transgenic
mouse models of Alzheimer's disease (K. Duff). 5.9. Modelling Down syndrome
in mice (M. Dierssen, M. Pritchard, C. Fillat, M. Arbonés, J.M. Aran, J.
Flórez, X. Estivill). 6. Ethical Considerations. 6.1. Genes and human
behavior: scientific and ethical implications of the human genome project
(J. Beckwith). 6.2. Ethical issues and psychiatric genetics (A.E. Farmer,
P. McGuffin). 6.3. Ethical implications of knock-out and transgenesis
techniques for animal research (L.M. Houdebine). Subject index.