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Phytochemical investigation of the leaves of Mentha piperita (L.) yielded a new compound, which is decarboxyrosemarinic acid galactoside. Formulate as 3', 4'-dihydroxy-beta-phenyl ethyl caffeate-4-(3'''-menthyl)-4'-beta-D-galacto pyranoside. 13C NMR, 1H NMR, FABMS, IR were used for structural characterization. Wound healing responses of this compound was evaluated from biochemical as well as biophysical parameters & analyze the role of isolated compound. The parameters studied included rate of wound contraction & the period of epithelialization in excision wound model. Tissues were removed on different days of intervals and subsequently analyzed for specific assays Tensile strength in incision wound model was assessed along with histopathological examinations. Compound-1 was found to increase the cellular proliferation and collagen synthesis at the wound site, as indicated by increases in amounts of DNA synthesized, protein content. Compound-1treatment was also shown to decrease the levels of lipid peroxides (LPs), while the activity of enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx),were significantly found to be increased when compare to the control.