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This is a monograph of the successful text An Introduction to Heavy metal tolerance analysis among gram negative bacteria from hospital aquatic environment. It has been extensively reviewed and covers large no of toxicity experiments methods, results and its discussion which help to student understanding of heavy metals resistance/ tolerance among gram negative bacteria from hospital wastewater. There are some new works on horizontal gene transfer in environmental condition. In addition screening of extra cellular enzymatic activity, Isolation of plasmid and conjugation among multi metal resistance isolates were studied. This monograph can be used, therefore as a complement to a standard theoretical as well as experimental text. Dr. Manzar Alam graduated from AMU Aligarh in 2010 with a B.Sc in Chemistry (Hons) and M.Sc, Ph.D in Microbiology from Integral University Lucknow in 2012 and 2017 respectively. He joined as Lab Instructor in the Department of Biosciences Integral University in 2013.