The formulation of herbal hair care product using extract of Argemone mexicana and Calotropis gigantea with special reference to their anti-microbial and antifungal activity had been reported first time. The prepared hair care formulation has antidandruff potential against Malassezia furfur, and antibacterial against E.coli, S.aureus, which are predominant causes for dandruff and hair fall. Prepared herbal hair care formulation is having advantage of non-synthetic base with least side effect and possible anti-inflammatory action as reported with these plant extracts. From the present work it can be concluded that prepared herbal formulation have a remarkable antidandruff efficacy in comparison with marketed antidandruff formulations, may be due to presence of following phytochemical constituents such as alkaloids, glycosides, tannins, steroids, and amino acids, detected in ethanolic extract of Argemone mexicana and Calotropis gigantea.