Written by the best researchers in the field, this timely book explores the use of high pressure in connection with spectroscopy as a research tool in biophysics and biochemistry. It covers subjects such as pressure tunning spectroscopy for investigating molecular interactions; high-pressure FT-IR studies of the secondary structure of proteins; structural, functional, and kinetic aspects of nucleic acid-protein complexes with pressure; sequence, salt, charge, and the stability of DNA at high pressure; steady-state enzyme kinetics at high pressure; membrane-free volume with bulky lipid…mehr
Written by the best researchers in the field, this timely book explores the use of high pressure in connection with spectroscopy as a research tool in biophysics and biochemistry. It covers subjects such as pressure tunning spectroscopy for investigating molecular interactions; high-pressure FT-IR studies of the secondary structure of proteins; structural, functional, and kinetic aspects of nucleic acid-protein complexes with pressure; sequence, salt, charge, and the stability of DNA at high pressure; steady-state enzyme kinetics at high pressure; membrane-free volume with bulky lipid concentration by regular distribution; and pressure and temperature induced inactivation of microorganisms.This book presents an overview of current research on pressure perturbations on enzyme kinetics, protein folding and structure, lipid bilayer structure and organization, lipid-protein interactions, DNA structure, and protein-ligand interactions.
* 1: Resolution of the Ambiguity of Vam't Hoff Plots by the Effect of Pressure Upon the Equilibrium * 2: Pressure Tunning Spectroscopy - A Tool for Investigating Molecular Interactions * 3: Use of Partial Molar Volumes of Model Compounds in the Interpretation of High-Pressure Effects on Proteins * 4: Pressure-tunning Spectroscopy of Proteins: Fourier Transform Infrared Studies in the Diamond Anvil Cell * 5: Temperature and Pressure-Induced Unfolding of a Mutant of Staphylococcal Nuclease A * 6: Pressure-Jump Relaxation Kinetics of Unfolding and Refolding Transitions of Staphylococcal Nuclease and Proline Isomerization Mutants * 7: High-Pressure FT-IR Studies of the Secondary Structure of Proteins * 8: High-Pressure NMR Studies of the Dissociation of Arc Repressor and the Cold Denaturation of Ribonuclease A * 9: Exploring Structural, Functional, and Kinetic Aspects of Nucleic Acid-Protein Complexes with Pressure: Nucleosomes and RNA Polymerase * 10: Pressure and Cold Denaturation of Proteins, Protein-DNA Complexes, and Viruses * 11: Sequence, Salt, Charge, and the Stability of DNA at High Pressure * 12: The Application of Pressure Relaxation to the Study of Substrate Binding to Cytochrome P versus Temperature, Pressure, and Viscosity * 13: Pressure Effects on the Ligand Binding Kinetics for Hemoproteins and Their Site-Directed Mutants * 14: Steady-State Enzyme Kinetics at High Pressure * 15: Transient Enzyme Kinetics at High Pressure * 16: The Effects of High Pressure on the Allosteric Properties of Phosphofructokinase from Escherichia coli * 17: Correlation Field Splitting of Chain Vibrations: Structure and Dynamics in Lipid Bilayers and Biomembranes * 18: High Pressure Effects on the Structure and Phase Behavior of Model Membranes Systems * 19: Membrane-Free Volume with Bulky Lipid Concentration by Regular Distribution: A Functionally Important Membrane Property Explored by Pressure Studies of Pyrene-Labelled Phosphatidylcholine * 20: NMR Studies of the Order and Dynamics of Dipalmitoylphosphatidylcholine Bilayers as a Function of Pressure * 21: The Effects of Increased Viscosity on the Function of Integral Membrane Proteins * 22: Pressure and Temperature Induced Inactivation of Microorganisms * 23: Osmotic and Hydrostatic Pressure as Tools to Study Molecular Recognition
* 1: Resolution of the Ambiguity of Vam't Hoff Plots by the Effect of Pressure Upon the Equilibrium * 2: Pressure Tunning Spectroscopy - A Tool for Investigating Molecular Interactions * 3: Use of Partial Molar Volumes of Model Compounds in the Interpretation of High-Pressure Effects on Proteins * 4: Pressure-tunning Spectroscopy of Proteins: Fourier Transform Infrared Studies in the Diamond Anvil Cell * 5: Temperature and Pressure-Induced Unfolding of a Mutant of Staphylococcal Nuclease A * 6: Pressure-Jump Relaxation Kinetics of Unfolding and Refolding Transitions of Staphylococcal Nuclease and Proline Isomerization Mutants * 7: High-Pressure FT-IR Studies of the Secondary Structure of Proteins * 8: High-Pressure NMR Studies of the Dissociation of Arc Repressor and the Cold Denaturation of Ribonuclease A * 9: Exploring Structural, Functional, and Kinetic Aspects of Nucleic Acid-Protein Complexes with Pressure: Nucleosomes and RNA Polymerase * 10: Pressure and Cold Denaturation of Proteins, Protein-DNA Complexes, and Viruses * 11: Sequence, Salt, Charge, and the Stability of DNA at High Pressure * 12: The Application of Pressure Relaxation to the Study of Substrate Binding to Cytochrome P versus Temperature, Pressure, and Viscosity * 13: Pressure Effects on the Ligand Binding Kinetics for Hemoproteins and Their Site-Directed Mutants * 14: Steady-State Enzyme Kinetics at High Pressure * 15: Transient Enzyme Kinetics at High Pressure * 16: The Effects of High Pressure on the Allosteric Properties of Phosphofructokinase from Escherichia coli * 17: Correlation Field Splitting of Chain Vibrations: Structure and Dynamics in Lipid Bilayers and Biomembranes * 18: High Pressure Effects on the Structure and Phase Behavior of Model Membranes Systems * 19: Membrane-Free Volume with Bulky Lipid Concentration by Regular Distribution: A Functionally Important Membrane Property Explored by Pressure Studies of Pyrene-Labelled Phosphatidylcholine * 20: NMR Studies of the Order and Dynamics of Dipalmitoylphosphatidylcholine Bilayers as a Function of Pressure * 21: The Effects of Increased Viscosity on the Function of Integral Membrane Proteins * 22: Pressure and Temperature Induced Inactivation of Microorganisms * 23: Osmotic and Hydrostatic Pressure as Tools to Study Molecular Recognition
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