This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.
This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.
Jinsong Zhou, Xi¿an Jiaotong University, Shannxi Province, China
Inhaltsangabe
Contents Chapter 1 Introduction 1.1 The contents and theories in Histochemistry 1.2 The basic requirements of Histochemistry methods
Chapter 2 The Tissue Preparation 2.1 Tissue collection 2.2 Fixation 2.3 Tissue washing-up, dehydration and clearance 2.4 Embedding 2.5 Sectioning 2.6 Adherence and mounting 2.7 Buffer
Chapter 3 The Carbohydrate and its Derivatives Histochemistry 3.1 Classification 3.2 Histochemistry methods
Chapter 4 The Nucleic Acid Histochemistry 4.1 DNA histochemical demonstration 4.2 Comparison demonstration of DNA and RNA Histochmistry
Chapter 5 The Lipid Histochemistry 5.1 Overview 5.2 Demonstration of lipids with physical methods 5.3 Demonstration of lipids with chemical methods
Chapter 6 Enzyme Histochemistry 6.1 Enzyme and its basic histochemical theory 6.2 Histochemistry for common enzymes
Chapter 7 The Basic Theory of Immunohistochemistry 7.1 Basic Immunology 7.2 Common markers and their detection 7.3 Basic conditions
Chapter 8 Common Methods Used in Immunohistochemistry 8.1 Principles 8.2 Immunofluorescence method 8.3 Immunoenzyme technique 8.4 Avidin-biotin method 8.5 Protein A method 8.6 Immunogold method and immune gold-silver method
Chapter 9 Specificity and Sensitivity of Immunohistochemistry 9.1 Specificity and specific staining 9.2 Control experiment 9.3 The methods to enhance immunohistochemical sensitivity
Chapter 10 The Double-Staining in Immunohistochemistry 10.1 Double-staining Immunohistochemistry on serial section 10.2 Immunofluorescence double labelling technique 10.3 Immunoenzyme double staining technique 10.4 Immunoenzyme-Immunofluorescence double staining 10.5 Immunoenzyme-Immunogold double staining
Chapter 11 The Lectin Histochemistry 11.1 Characteristics and application of lectin 11.2 Application of lectin Histochemistry
Chapter 12 The progress of in Situ Display 12.1 The envision method 12.2 The catalysed signal amplification method 12.3 The ferric oxide alternative method for HRP
Chapter 13 In Situ Hybridization Immunohistochemistry 13.1 Basic principle 13.2 Probe preparation 13.3 Procedure of in situ hybridization histochemistry 13.4 Factors affecting in situ hybridization 13.5 Control test
Chapter 14 In Situ Polymerase Chain Reaction Technique 14.1 Basic principle 14.2 Basic types 14.3 Procedure 14.4 Application of in situ PCR technology
Chapter 15 Electron Microscopic Cytochemical Technique 15.1 Electron microscopic enzyme cytochemistry technology 15.2 Electron microscopic Immunocytochemical technique
Chapter 16 The Quantitative Assay of Histochemistry Results 16.1 The photomicrography 16.2 The image analyzer 16.3 The flow cytometry 16.4 The laser scanning confocal microscopy
Contents Chapter 1 Introduction 1.1 The contents and theories in Histochemistry 1.2 The basic requirements of Histochemistry methods
Chapter 2 The Tissue Preparation 2.1 Tissue collection 2.2 Fixation 2.3 Tissue washing-up, dehydration and clearance 2.4 Embedding 2.5 Sectioning 2.6 Adherence and mounting 2.7 Buffer
Chapter 3 The Carbohydrate and its Derivatives Histochemistry 3.1 Classification 3.2 Histochemistry methods
Chapter 4 The Nucleic Acid Histochemistry 4.1 DNA histochemical demonstration 4.2 Comparison demonstration of DNA and RNA Histochmistry
Chapter 5 The Lipid Histochemistry 5.1 Overview 5.2 Demonstration of lipids with physical methods 5.3 Demonstration of lipids with chemical methods
Chapter 6 Enzyme Histochemistry 6.1 Enzyme and its basic histochemical theory 6.2 Histochemistry for common enzymes
Chapter 7 The Basic Theory of Immunohistochemistry 7.1 Basic Immunology 7.2 Common markers and their detection 7.3 Basic conditions
Chapter 8 Common Methods Used in Immunohistochemistry 8.1 Principles 8.2 Immunofluorescence method 8.3 Immunoenzyme technique 8.4 Avidin-biotin method 8.5 Protein A method 8.6 Immunogold method and immune gold-silver method
Chapter 9 Specificity and Sensitivity of Immunohistochemistry 9.1 Specificity and specific staining 9.2 Control experiment 9.3 The methods to enhance immunohistochemical sensitivity
Chapter 10 The Double-Staining in Immunohistochemistry 10.1 Double-staining Immunohistochemistry on serial section 10.2 Immunofluorescence double labelling technique 10.3 Immunoenzyme double staining technique 10.4 Immunoenzyme-Immunofluorescence double staining 10.5 Immunoenzyme-Immunogold double staining
Chapter 11 The Lectin Histochemistry 11.1 Characteristics and application of lectin 11.2 Application of lectin Histochemistry
Chapter 12 The progress of in Situ Display 12.1 The envision method 12.2 The catalysed signal amplification method 12.3 The ferric oxide alternative method for HRP
Chapter 13 In Situ Hybridization Immunohistochemistry 13.1 Basic principle 13.2 Probe preparation 13.3 Procedure of in situ hybridization histochemistry 13.4 Factors affecting in situ hybridization 13.5 Control test
Chapter 14 In Situ Polymerase Chain Reaction Technique 14.1 Basic principle 14.2 Basic types 14.3 Procedure 14.4 Application of in situ PCR technology
Chapter 15 Electron Microscopic Cytochemical Technique 15.1 Electron microscopic enzyme cytochemistry technology 15.2 Electron microscopic Immunocytochemical technique
Chapter 16 The Quantitative Assay of Histochemistry Results 16.1 The photomicrography 16.2 The image analyzer 16.3 The flow cytometry 16.4 The laser scanning confocal microscopy
References
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