Tim D. Hewitson (ed.) / Ian A. Darby

Histology Protocols

Herausgegeben:Hewitson, Tim D.; Darby, Ian A.

• Gebundenes Buch

Produktbeschreibung

Somuchofwhatweknowaboutthepathogenesisofhumandiseasehascomefromthe systematic and careful study of histological material. Indeed, every internal medicine discipline has its landmark papers describing the clinico-pathological correlations. However, increasingly, it is molecular and cellular biology that provides the necessary mechanistic insights. For many years, it was thought that the two skill sets were mutually exclusive, but we hope that this book shows that this is not necessarily so. Implicitinthescienceofhistologyisthepreservationandarchivingoftissue.PartIof the book concentrates on the preparation of tissue, providing an overview of fixation, embedding, and processing (Chapter 1), and in Chapters 2 and 3, the required techniques for the retrieval of RNA from histological sections. Both routine and specialist histological staining techniques are provided in Part II. These include pro- cols for immuno (Chapters 4-7), lectin (Chapter 8), and hybridization (Chapter 9) histochemistry,histologicalstaining (Chapters10and11),aswellasspecificmethods for the in situ identification of hypoxia (Chapter 12) and apoptosis (Chapter 13). Finally, Part III details advances in imaging (Chapters 14-16) and image analysis (Chapter 17). It is hoped that this volume will provide molecular biologists with the basic his- chemical techniques and histologists with the molecular techniques to realise the potential of their resource. We are indebted to the authors for their generosity in sharing these protocols.

Produktdetails

• Methods in Molecular Biology 611
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 12083312, 978-1-60327-344-2
• 2010 edition
• Seitenzahl: 229
• Erscheinungstermin: 16. Dezember 2009
• Englisch
• Abmessung: 254mm x 178mm x 14mm
• Gewicht: 585g
• ISBN-13: 9781603273442
• ISBN-10: 1603273441
• Artikelnr.: 27028584

Inhaltsangabe

Tissue Processing.- Tissue Preparation for Histochemistry: Fixation, Embedding, and Antigen Retrieval for Light Microscopy.- An Optimized RNA Extraction Method from Archival Formalin-Fixed Paraffin-Embedded Tissue.- Laser-Capture Microdissection and Pressure Catapulting for the Analysis of Gene Expression in the Renal Glomerulus.- Staining Techniques.- Immunofluorescence Detection of the Cytoskeleton and Extracellular Matrix in Tissue and Cultured Cells.- Double Immunohistochemistry with Horseradish Peroxidase and Alkaline Phosphatase Detection Systems.- Retrogradely Transported Neuronal Tracers Combined with Immunohistochemistry Using Free-Floating Brain Sections.- High-Pressure Freezing, Chemical Fixation and Freeze-Substitution for Immuno-electron Microscopy.- Lectin Histochemistry for Light and Electron Microscopy.- Duplex In Situ Hybridization in the Study of Gene Co-regulation in the Vertebrate Brain.- Special Stains for Extracellular Matrix.- Active Staining of Mouse Embryos for Magnetic Resonance Microscopy.- Immunohistochemical Detection of Tumour Hypoxia.- In Situ Localization of Apoptosis Using TUNEL.- Imaging Techniques.- Use of Confocal Microscopy for Three-Dimensional Imaging of Neurons in the Spinal Cord.- High-Resolution Confocal Imaging in Tissue.- Software-Based Stacking Techniques to Enhance Depth of Field and Dynamic Range in Digital Photomicrography.- Image Analysis and Quantitative Morphology.

Rezensionen

From the reviews:

"This slim volume aims to provide molecular biologists with the basic histochemical techniques and histologists with the molecular techniques to realise their potential of their resource. ... I enjoyed reading this book ... . this book is not particularly expensive and is so comprehensive and well written that I would certainly recommend it to anyone thinking of setting up a research project but uncertain what sort of method to use. It would also be a valuable asset to any departmental library ... ." (Anne Patricia Campbell, Bulletin of the Royal Society of Pathologists, Issue 154, April, 2011)

"Reports the basic techniques of immunohistochemistry and molecular biology used to optimize resources and obtain accurate results. ... This book is recommended to students and researchers using the laboratory techniques on histology described. Updated protocols are described by renowned scientists in the area using understandable language while details of possible interference are given for each technique. Tim D. Hewiston and Ian A. Darby have undertaken several studies on histology, many of which were conducted in collaboration with the authors contributing to this book." (Rui Curi, Brazilian Journal of Pharmaceutical Sciences, Spring, 2011)

"This volume in the Methods in Molecular Biology series provides a compendium of proven protocols for modern histology. ... Written for basic science and clinical researchers interested in mapping the location and distribution of biomarkers, the book also will be appreciated by lecturers and students specializing in biomedical technology, histology, and clinical laboratory medicine. ... The book is well written and carefully edited, and will be a welcome resource for all basic science and clinical researchers studying modern cell biology." (Bruce A. Fenderson, Doody's Review Service, June, 2010)