Hormone Assays in Biological Fluids

Herausgegeben:Wheeler, Michael J.; Fraser, William D.; Hutchinson, J. S. Morley

• Broschiertes Buch

Produktbeschreibung

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Produktdetails

• Methods in Molecular Biology 324
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 978-1-61737-299-5
• Seitenzahl: 258
• Erscheinungstermin: 10. November 2010
• Englisch
• Abmessung: 229mm x 152mm x 15mm
• Gewicht: 412g
• ISBN-13: 9781617372995
• ISBN-10: 1617372994
• Artikelnr.: 32201956

Inhaltsangabe

Immunoassay Techniques.- High-Performance Liquid Chromatography for Hormone Assay.- Gas Chromatography-Mass Spectrometry.- An Introduction to PCR Techniques.- Methods for the Investigation of Thyroid Function.- Assays for LH, FSH, and Prolactin.- Adrenocorticotrophic Hormone and Related Peptides.- Measurement of Glucocorticoids in Biological Fluids.- Urinary Steroid Profiling.- Measurement of Aldosterone in Blood.- Measurement of Plasma Renin Activity.- Measurement of Androgens.- Measurement of Gut Hormones in Plasma.- Measurement of Melatonin and 6-Sulphatoxymelatonin.

Rezensionen

From the reviews:

"Interest in hormone diagnostics has developed dramatically over the last decades ... . In summary, this book is written by knowledgeable experts in the field of hormone analysis in biological fluids ... . It provides a valuable collection of assays, described in a practical way, so they can be rapidly implemented by laboratorians looking for hormone assays suitable for research purposes or as alternatives to commercial immunoassays." (Linda M. Thienpont, Clinical Chemistry, Vol. 52 (9), 2006)

"...a wonderful resource for all thoe who, at some point, face decisions about the most suitable hormone assay for a particular situation." -TRENDS in Endocrinology and Metabolism