HPLC of Peptides and Proteins

Methods and Protocols
Herausgegeben:Aguilar, Marie-Isabel

• Broschiertes Buch

Produktbeschreibung

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Produktdetails

• Methods in Molecular Biology 251
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 978-1-61737-286-5
• Seitenzahl: 414
• Erscheinungstermin: 10. November 2010
• Englisch
• Abmessung: 235mm x 154mm x 30mm
• Gewicht: 630g
• ISBN-13: 9781617372865
• ISBN-10: 1617372862
• Artikelnr.: 32201334

Inhaltsangabe

Modes of HPLC.- HPLC of Peptides and Proteins.- Reversed-Phase High-Performance Liquid Chromatography.- Ion-Exchange Chromatography.- High-Performance Hydrophobic Interaction Chromatography.- Gel Filtration Chromatography.- Hydrophilic Interaction Chromatography.- Immobilized Metal Ion Affinity Chromatography of Proteins.- Immunoaffinity Chromatography of Proteins.- Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins.- Preparative Methodologies.- Capillary Separations.- Micropreparative HPLC of Peptides and Proteins.- Multidimensional HPLC Purification of Proteins.- Analytical High-Performance Liquid Chromatography.- Prep/Semiprep Separations of Peptides.- Large-Scale Protein Chromatography.- Applications.- Proteolytic Peptide Mapping.- Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation.- Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry.- HPLC in the Analysis of Peptide Metabolism.- Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells.- Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques.- HPLC and Mass Spectrometry of Intrinsic Membrane Proteins.- IgG Purification.- DNA-Binding Proteins.- Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC.- Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry.- Proteome Analysis.

Rezensionen

"...a showcase of state-of-the-art methodologies in the application of HPLC to protein/peptide studies...can be used by beginners and intermediate experts in this field to develop separation and analytical protocols...all scientists dealing with peptides and proteins can benefit from reading this book." - Proteomics

"...this book is well written...a very usable laboratory companion or reference guide for researchers in pursuit of peptide and protein characterizations." - Biomedical Chromatography