Human polyomavirus, JCV, virus-like particle as a gene delivery vector - Chen, Li-Hsien
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In the current study, a novel in vivo DNA packaging method using the JCV VLP was employed to obtain more highly efficient gene transfer. A reporter gene, the green fluorescence protein (gfp), and a suicide gene, the herpes simplex virus thymidine kinase (tk), were encapsidated into VLPs in E. coli. The VLP was used to specifically target human colon carcinoma (COLO-320 HSR) cells in a nude mouse model. Intra-peritoneal administration of ganciclovir (GCV) in the tk-VLP treated mice greatly reduced tumor volume. These findings suggest that it will be possible to develop the JCV VLP as a gene delivery vector for human colon cancer therapy in the future.…mehr

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Produktbeschreibung
In the current study, a novel in vivo DNA packaging method using the JCV VLP was employed to obtain more highly efficient gene transfer. A reporter gene, the green fluorescence protein (gfp), and a suicide gene, the herpes simplex virus thymidine kinase (tk), were encapsidated into VLPs in E. coli. The VLP was used to specifically target human colon carcinoma (COLO-320 HSR) cells in a nude mouse model. Intra-peritoneal administration of ganciclovir (GCV) in the tk-VLP treated mice greatly reduced tumor volume. These findings suggest that it will be possible to develop the JCV VLP as a gene delivery vector for human colon cancer therapy in the future.
Autorenporträt
Doctor of Science in Department of Chemistry and Biochemistry at National Chung Cheng University. Postdoctoral fellowship at National Taiwan University Hospital Clinical Trial Center. Major in Screening the biomarker of the gastric cancer with gastric cancer specimen and Bioelectronics/biochip system development for the application of life science. The current position is the Assistant Professor in Department of Cosmetic Applications and Management, Cardinal Tein Junior College of Healthcare and Management. The teaching courses are about the Biochemistry.