Human Stem Cell Technology and Biology

A Research Guide and Laboratory Manual
Herausgeber: Stein, Gary S; Vazquez, Priscilla; Smith, Kelly P; Shi, Meng-Jiao; Luong, Mai X; Borowski, Maria

• Gebundenes Buch

Produktbeschreibung

The first comprehensive multimedia training manual for stem cell education and research The first resource of its kind, Human Stem Cell Technology and Biology presents laboratory techniques and protocols that have proven to be effective for the culture of pluripotent stem cells, as well as current perspectives in the field. Edited by a team of professionals at the forefront of discovery and innovation in stem cell research, education, and development of infrastructure, this authoritative multimedia training manual distinguishes itself by integrating readily accessible text, electronic, and video components to explain the biology, technology, and applications of human embryonic stem cells. The first section of the text begins with an overview of the research that established the foundation for current initiatives in stem cell research; it goes on to describe the best methods for researching and obtaining pluripotent cell lines. The second section provides the skills and techniques necessary for the culture, maintenance, and characterization of pluripotent stem cells. This section has been developed as a multimedia stem cell course in order to present the most comprehensive instruction possible. The third section presents an in-depth perspective on the current status of the stem cell research field, and the fourth section explores applications, describing the most recent advances in research and the interface with gene therapy, tissue engineering, and drug testing. Features of this one-of-a-kind reference include: * Introductory chapters that provide background information to scientists entering the field * State-of-the-art, contemporary perspectives written by international leaders in the field * Printable laboratory protocols in PDF form, accessible on the book's Web site * Online updates of the rapidly developing strategies and research protocols that support stem cell research Human Stem Cell Technology and Biology is an invaluable reference and laboratory manual for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field. Whether it is used to design a laboratory curriculum, workshop, or training course, or simply to assist in performing research in stem cell biology, this volume is a must-have resource of protocols, information, and perspectives for professional laboratories, colleges, universities, and vocational schools.

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Produktdetails

• Verlag: Wiley
• Seitenzahl: 440
• Erscheinungstermin: 4. Januar 2011
• Englisch
• Abmessung: 289mm x 222mm x 29mm
• Gewicht: 1278g
• ISBN-13: 9780470595459
• ISBN-10: 0470595450
• Artikelnr.: 29337700

Autorenporträt

Gary Stein, lead Editor for this publication, is currently Professor & Chairman of the Deartment of Cell Biology at the University of Massachusetts Medical School (Worcester, MA); Deputy Director of the UMASS Memorial Cancer Center; Appointed Haidak Distinguished Prof. & Chair of Cell Biology; Interim Director of the Center for Stem Cell Biology and Regenerative Medicine; and Interim Director of the UMASS Cancer Center. He is Executive Editor of Journal of Cellular Biochemistry, Journal of Cellular Physiology, Critical Reviews in Eukaryotic Gene expression, and Molecular Biology Reports, and has served on numerous Editorial Boards. He has published over 700 full length research articles, and has authored numerous books.

Inhaltsangabe

Foreword.
Preface.
Acknowledgments.
Editors and Contributors.
SECTION I: INTRODUCTION.
1 Introduction to Pluripotent Stem Cells: Biology and Applications (Maria
Borowski and Gary S. Stein).
2 Researching and Obtaining Established Stem Cell Lines (Mai X. Luong,
Kelly P. Smith, and Gary S. Stein).
SECTION II: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: PLURIPOTENT STEM
CELL CULTURE.
3 Basics of Cell Culture (Alicia Allaire, Mai X. Luong, and Kelly P. Smith
).
4 The Stem Cell Laboratory (Alicia Allaire, Mai X. Luong, and Kelly P.
Smith).
5 Reagent Preparation (Alicia Allaire, Mai X. Luong, and Kelly P. Smith).
6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic
Stem Cells (Meng-Jiao Shi, Maria Borowski, and Kimberly Stencel).
7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts (Meng-Jiao Shi,
Maria Borowski, and Kimberly Stencel).
8 Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells (
Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski).
9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic
Fibroblast Plates (Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski).
10 Harvesting Human Embryonic Stem Cells for Cryopreservation (Meng-Jiao
Shi, Kimberly Stencel, and Maria Borowski).
11 Human Embryonic Stem Cell Culture on BD Matrigel(TM)with mTeSR®1 Medium
(Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski).
SECTION III: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: CHARACTERIZATION
OF PLURIPOTENT STEM CELLS.
12 Defining Pluripotency (Kelly P. Smith and Mai X. Luong).
13 Characterization of Human Embryonic Stem Cells by Immunofluorescence
Microscopy (Shirwin M. Pockwinse and Prachi N. Ghule).
14 Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry (
Meng-Jiao Shi, Kimberly Stencel, and Maria Borowski).
15 Characterization of Human Embryonic Stem Cell Gene Expression Using
Reverse Transcription Polymerase Chain Reaction (Alicia Allaire, Kelly P.
Smith, and Mai X. Luong).
16 Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body
Formation (Shirwin M. Pockwinse and Prachi N. Ghule).
17 Characterization of Human Embryonic Stem Cells by Cytogenetics:
Karyotyping and Fluorescence In Situ Hybridization (Shirwin M. Pockwinse,
Prachi N. Ghule and Anne Higgins).
18 High-Resolution Chromatin Immunoprecipitation Assay (Beatriz
Perez-Cadah¿a, Bojan Drobic, and James R. Davie).
19 Assaying Pluripotency Via Teratoma Formation (M. William Lensch and Tan
A. Ince).
SECTION IV: PERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES.
20 Genomic Analysis of Pluripotent Stem Cells (David Lapointe).
21 Proteomic Analysis of Human Pluripotent Cells (Andy T. Y. Lau, Yan-Ming
Xu, and Jen-Fu Chiu).
22 Bioinformatics Strategies for Understanding Gene Expression in Human
Pluripotent Cells (Gustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho,
Burak Kutlu, and Qiang Tian).
23 Epigenetic Analysis of Pluripotent Cells (Mojgan Rastegar, Genevieve P.
Delcuve, and James R. Davie).
24 Differentiation of Pluripotent Stem Cells: An Overview (Jeremy Micah
Crook).
25 Cellular Reprogramming: Current Technology, Perspectives, and Generation
of Induced Pluripotent Cells (Tanja Dominko).
SECTION V: APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS.
26 Human Pluripotent Cells: The Biology of Pluripotency (Li-Fang Chu and
Thomas P. Zwaka).
27 Human Pluripotent Cells for Regenerative Medicine: Potential
Applications for Regenerative Medicine (Christopher C. Ford and Darrell N.
Kotton).
28 Therapeutic Applications of Human Embryonic Stem Cells (Shi-Jiang Lu,
Irina Klimanskaya, Edmund Mickunas, and Robert Lanza).
29 Human Stem Cells for Drug Screening, Toxicity, Specificity, and
Off-Target Effects (Arnaud Lacoste and Mark Burcin).
30 Interfacing Stem Cells with Gene Therapy (Christian Mueller and Terence
R. Flotte).
31 Tissue Engineering for Stem Cell Mediated Regenerative Medicine (Janet
Zoldan, Thomas P. Kraehenbuehl, Abigail K. R. Lytton-Jean, Robert S.
Langer, and Daniel G. Anderson).
Glossary.
Index.

Rezensionen

"The topic is intrinsically fascinating and clinically relevant ...I recommend this book for novice and experienced scientistsinterested in stem cell biology. It will serve as an excellentreference manual and practical laboratory workbook." (Doody's, 17February 2012)

"We would recommend this readable and well-organised book to alllaboratories dealing with human ES cells ... This book will act asan excellent guide." (Development Journal, 1 December 2011)