Hybridoma Technology in the Biosciences and Medicine
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I. The Production and Improvement through Genetic Engineering of Monoclonal Antibodies.- 1. Monoclonal Antibody Strategy and Techniques.- 2. Strategies for Production of Human Monoclonal Antibodies.- 3. The Antibody Combining Site.- 4. The Generation of Better Monoclonal Antibodies through Somatic Mutation.- 5. Switching the Isotype of Monoclonal Antibodies.- 6. The Analysis of Structural Diversity in the Antibody Response.- 7. Expressions of Cloned Immunoglobulin Genes.- II. Monoclonal Antibodies in Biomedical Research: Principles and Applications.- 8. The Immunochemistry of Monoclonal Antibo...
I. The Production and Improvement through Genetic Engineering of Monoclonal Antibodies.- 1. Monoclonal Antibody Strategy and Techniques.- 2. Strategies for Production of Human Monoclonal Antibodies.- 3. The Antibody Combining Site.- 4. The Generation of Better Monoclonal Antibodies through Somatic Mutation.- 5. Switching the Isotype of Monoclonal Antibodies.- 6. The Analysis of Structural Diversity in the Antibody Response.- 7. Expressions of Cloned Immunoglobulin Genes.- II. Monoclonal Antibodies in Biomedical Research: Principles and Applications.- 8. The Immunochemistry of Monoclonal Antibodies: Studies on One Immunologic Supergene (HLA) with the Products of Another.- 9. Purification of Membrane Glycoproteins with Monoclonal Antibody Affinity Columns.- 10. Transferrin Receptor.- 11. Functional and Structural Interrelationships among the Mac-1, LFA-1 Family of Leukocyte Adhesion Glycoproteins, and their Deficiency in a Novel Heritable Disease.- 12. Demonstration of Receptor Function of Membrane Proteins by Selection and Immobilization with Monoclonal Antibodies.- 13. Production of Rat × Mouse Hybridomas for the Study of the Nicotinic Acetylcholine Receptor.- 14. Sperm Surface Domains.- 15. The Cytoskeleton.- 16. Monoclonal Antibodies as Molecular Probes of the Nervous System.- 17. Application of Monoclonal Antibodies to Issues of Plasma Lipoprotein Structure and Function.- 18. Enzyme Active Sites.- 19. Monoclonal Antibodies in Studies of the Hong Kong Influenza Hemagglutinin.- 20. Virus Receptors on Somatic and Immune Cells.- III. Uses of Antibodies in Recombinant DNA Technology.- 21. Designing and Using Site-Specific Antibodies to Synthetic Peptides.- 22. Gene Transfer as a General Approach to Facilitate the Recombinant DNA Cloning of Surface Antigen Genes.- 23.Amplification and Molecular Cloning of Transfected Genes.- 24. Screening ?gt11 Expression Libraries with Antibody Probes.- 25. Purification of Low-Abundance Messenger RNAs by Polysome Isolation with Polyclonal Antibodies.- 26. Purification of Messenger RNA by Polysome Isolation with Monoclonal Antibodies.- 27. Expression of Foreign Proteins on the Surface of Xenopus laevis Oocytes.- IV. Monoclonal Antibodies in Diagnosis and Therapy.- 28. Antibodies in Vivo.- 29. Human Complement-Fixing Antibodies in Transplantation.- 30. Manipulation of T-Cell Populations to Abrogate Allograph Rejection.- 31. Serotherapy and Bone Marrow Transplantation.- V. T-Cell Clones and Their Products.- 32. The Murine T-Cell Antigen: Ia Receptor: Functional and Structural Analysis with a Monoclonal Antireceptor Antibody.- 33. Functional T-Cell Hybridomas.- 34. The Mechanisms That Control the Proliferation and Activity of Cytolytic T Lymphocytes.- 35. Human Cytolytic T-Lymphocyte Clones and Their Function-Associated Cell Surface Molecules.- 36. The Use of Mouse Lymphocyte Clones to Define T-Cell Gene Products and Analyze Lymphocyte Gene Activation.- 37. Interleukin 1, Interferon Gamma, and the Modulation of Macrophage-T Cell Interactions.
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