Dennis Kuo

Hydrodynamic Analysis of Groucho Q-Domain

Physical Biochemistry techniques reveals an unexpected shape

• Broschiertes Buch

Produktbeschreibung

The Groucho (Gro)/Transducin-like-enhancer of split (TLE) family of corepressors plays a significant role in the regulation of many cellular processes. Having no intrinsic DNA-binding activity, Gro/TLE interacts with various DNA-bound repressor proteins to repress transcription. Gro/TLE possesses a conserved region known as the Q-domain, and this region has been shown to direct the oligomerization of Gro/TLE, which is required for repression. Although the Q-domain is required for oligomerization, its structural properties have never been fully characterized. Here, the Q-domain was tagged with a small ubiquitin-related modifier protein (SUMO) to alleviate aggregation observed with the untagged Q-domain. Size exclusion chromatography (Gel Filtration) and analytical ultracentrifugations were performed. Results show that SUMO-Q oligomerizes predominantly as a tetramer. However, significant heterogeneity is observed at high salt concentrations. Analysis of the hydrodynamic data suggest a markedly non-spherical structure.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 80
• Erscheinungstermin: 28. November 2010
• Englisch
• Abmessung: 220mm x 150mm x 5mm
• Gewicht: 127g
• ISBN-13: 9783843374231
• ISBN-10: 3843374236
• Artikelnr.: 32589986

Autorenporträt

Dennis Kuo received his Bachelor of Science degree with a minor in music history at University of California, Los Angeles. He received his Master of Science degree in Biochemistry and Molecular Biology under the guidance of Professor Albert Courey.