Immunochemistry of Proteins

Volume 1
Herausgegeben von Atassi, M. Zouhair

• Broschiertes Buch

Produktbeschreibung

The structural features responsible for the immunogenicity of certain parts of native protein molecules have been of interest to immunochemists and protein chemists for over three decades. Following the early work of Land steiner in 1942, which showed that peptide fragments from silk fibroin exhibited an inhibitory activity toward the reaction of the protein with its antibodies, fragments from many other protein systems have been isolated and studied. However, no concerted effort was (or could be) devoted to the elucidation of the complete antigenic structure of a protein. In order for these endeavors to be successful and meaningful, knowledge of both the amino acid sequence and the detailed three-dimensional structure of the protein is necessary. Such information was not available for a protein until early in the 1960s. This and the fact that protein chemistry was not in fact sufficiently developed early in the 1960s to enable the successful completion of the entire antigenic structure of a protein were major contributing factors for the slow progress in this field. Determination of the antigenic structures of proteins therefore posed a chemical challenge of enormous proportions. For these reasons, many investigators diverted their attention to study of the immunochemistry of homo- or mixed amino acid polymers in the hope that the information derived from these systems might prove useful in the understanding of the immunochemistry of proteins.

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Produktdetails

• Verlag: Springer / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4613-4192-5
• 1977
• Seitenzahl: 508
• Erscheinungstermin: 19. Oktober 2011
• Englisch
• Abmessung: 235mm x 155mm x 28mm
• Gewicht: 761g
• ISBN-13: 9781461341925
• ISBN-10: 1461341922
• Artikelnr.: 40735309

Inhaltsangabe

1 Chemical Modification and Cleavage of Proteins and Chemical Strategy in Immunochemical Studies of Proteins M. Z. Atass.- I. Introduction.- II. Usefulness and Limitations.- III. Choice of Reaction and Evaluation of Results.- IV. Modification Reactions of Various Side Chains.- V. Usefulness, Limitations, and Evaluation of the Results.- VI. Choice of Reaction.- VII. Cleavage Reactions.- VII. References.- 2 Influence of Conformation on Immunochemical Properties of Proteins.- I. Introduction.- II. Factors Responsible for Protein Folding.- III. Dependence of Conformation on the Whole Amino Acid Sequence: Conformation of Unique Protein Fragments.- IV. Use of Immunochemical Methods as a Conformational Probe.- V. Influence of Conformational Changes on Immunochemical Behavior.- A. Conformational Changes Induced by Modification of Nonimmunogenic Prosthetic Groups.- VI. Conclusion.- VII. References.- 3 Investigation of Immunochemical Reactions by Fluorescence Polarization.- I. Introduction.- II. Symbols.- III. Equations Relating Fluorescence Polarization and Intensity to Concentration.- IV. Equilibrium Equations.- V. Kinetic Equations.- VI. Relaxation Methods.- VII. Results Obtained on Immunochemical Reactions by Means of Fluorescence Polarization and Intensity Measurements.- VIII. What Has Been Learned from Fluorescence Polarization Measurements on Antigen-Antibody and Hapten-Antibody Reactions?.- IX. References.- 4 In Vitro Immune Responses of Lymphoid Cell Populations to Proteins and Peptides.- I. Introduction.- II. In Vitro Consequences of the Reaction of Proteins or Peptides with Lymphoid Cell Populations.- III. Induction and Regulation of in Vitro Immune Responses by Soluble Factors.- IV. Discussion.- V. References.- 5 Immunochemistry of Encephalitogenic Protein.- I. Introduction.- II. Nature and Properties of Encephalitogenic Protein.- III. Antigenic Determinants with Disease-Inducing Properties.- IV. Antigenic Determinants Connected with Protection against Disease.- V. Antigenic Determinants Connected with Cell-Mediated Immunity.- VI. Antigenic Determinants for the Humoral Immune Response...- VII. Concluding Remarks.- VIII. References.- 6 Immunochemistry of Collagen.- I. Chemical and Structural Considerations of Collagen.- II. Immunochemistry of Collagen.- III. Cell-Mediated Immunity to Collagen.- IV. Genetic Control of the Immune Response to Collagen.- V. Antibodies to Procollagen.- VI. Applications of Collagen Immunochemistry to Medical Research.- VII. References.- 7 Histocompatibility Antigens.- I. The HL-A System of Man.- II. Alloantigens of the Mouse.- III. Purification Methods for H-2 and HL-A Alloantigens.- IV. Chemical Nature of H-2 and HL-A Antigens.- V. Conclusion.- VI. References.- Author Index.