Stefan H.E. Kaufmann / Dieter Kabelitz (Volume ed.)

Immunology of Infection

Volume 25
Herausgegeben:Kaufmann, Stefan H.E.; Kabelitz, Dieter

• Broschiertes Buch

Produktbeschreibung

Established for almost 30 years, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting-edge protocols to directly benefit your research.

Immunology of Infection, edited by two of the foremost figures in the field, presents the most appropriate, up-to-date techniques in the detail you require. The layout is structured for ease of reference, and the volume will be essential reading for all researchers working in microbiology, immunology, virology, mycology, and parasitology.The new volume provides a carefully selected collection of immunological techniques for the microbiologist wishing to study host-pathogen relationships in vivo and in vitro. This multi-authored book has succeeded in bringing together experts from various fields of molecular and cellular immunology who provide ready-to-use recipes for the ex vivo and in vitro analysis of anti-infective immunity.

Produktdetails

• Methods in Microbiology Volume 25
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0580-9517(08)X7034-5
• 1998.
• Seitenzahl: 705
• Erscheinungstermin: 24. Juni 1998
• Englisch
• Abmessung: 246mm x 183mm x 37mm
• Gewicht: 1324g
• ISBN-13: 9780124023055
• ISBN-10: 0124023053
• Artikelnr.: 22308278

Inhaltsangabe

Introduction:
S.H.E. Kaufmann and D. Kabelitz, The Immune Response to Infectious Agents.
General Methods:
A. Scheffold and A. Radbruch, Phenotyping and Separation of Leukocyte Populations.
D. Kabelitz and K. Pechold, Measurement of Cellular Proliferation (³H-TdR, MTT, Absolute Cell Numbers by SCUD).
Murine Models:
H. Mossmann, Management of Infected and Immunocompromised Animals.
C.J. Czuprynski and J.F. Brown, In Vitro Analysis.
W. Born, M. Tsuji, C. Cady, M. Lahn, A. Mukasa, C. Reardon, and R. O'Brien, Establishment of Murine T Cell Lines, T Cell Clones, Hybridomas and Transfectomas.
D.H. Busch and E.G. Pamer, Killer Cell Assays.
R. Haworth and S. Gordon, Isolation of Professional Phagocytes and Measuring their Functions: Murine Macrophages in Vitro.
M. Mielke, In Vivo Analyses.
S. Ehlers and U. Seitzer, Measuring Immune Responses in Vivo.
A.D. Roberts, J.T. Belisle, A.M. Cooper, and I.M. Orme, Specific Models.
P. Kropf, K. Brunson, R. Etges, and I. Muller, The Leishmaniasis Model.
J.B. Ulmer, J.J. Donnelly, D.L. Montgomery, and M.A. Liu, Art and Science of DNA Vaccines.
K. Lövgren-Bengtsson, Preparation and Use of Adjuvants.
Human System:
J. Banchereau, Isolation and Propagation of Dendritic Cells.
E. Märker-Hermann and R. Duchmann, Isolation of T Cells and Establishment of T Cell Lines and Clones.
H. Fickenscher and B. Fleckenstein, Growth-Transformation of Human T Cells.
G. Pawelec, E. Mariani, R. Offringa, C.J.M. Melief, and R. Solana, Generation and Characterization of Killer Cells.
H. Yssel and F. Cottrez, Measuring Human Cytokine Responses.
U. Seitzer and J. Gerdes, Measuring Immune Responses in Situ: Immunohistology and in Situ Hybridization.
S.W. Krause, M. Kreutz, and R. Andreesen, Isolation, Characterization, and Cultivation of Human Monocytes and Macrophages.

---

Introduction
The immune response to infectious agents
General Methods
Phenotyping and separation of leukocyte populations based on affinity
labelling
Cytometric cytokine secretion assay: detection and isolation of cytokine
secreting T cells
Measurement of cellular proliferation
Use of bioinformatics to predict MHC ligands and T-cell epitopes:
application to epitope driven vaccine design
The use of tetramers in the quantitative analysis of T cell responses
Using microarrays for studying the host transcriptional response to
microbial infection
Genome-wide expression profiling of intracelllular bacteria: the
interaction of Mycobacterium tuberculosis with macrophages
Murine Models
Management of immuno-compromised and infected animals
In Vitro Analysis
Isolation and preparation of lymphocytes from infected animals for in vitro
analysis
Isolation and development of murine T-lymphocyte hybridomas and clonal cell
lines
Killer cell assays
Quantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry
and Reverse Transcriptase-PCR Methods
Isolation of and measuring the function of professional phagocytes: murine
macrophages
In vivo Analysis
The immune response in mice challenged with bacterial infections
Measuring immune responses in vivo
Specific Models
Murine model of tuberculosis
The leishmaniasis model
Animal models: murine cytomegalovirus
DNA vaccines: fundamentals and practice
Preparation and use of adjuvants
Human Systems
Isolation and propagation of human dendritic cells
Isolation of T cells and establishment of T-cell lines and clones
Growth-transformation of human T cells
Generation and characterisation of human killer cells
Measuring human cytokine responses
Measuring immune responses in situ: immunofluorescent and immunoenzymatic
techniques
Isolation, characterisation and cultivation of
human monocytes and macrophages