TB infection causes a variety of clinical conditions ranging from lifelong symptomatic infection to public illness with increasingly severe tissue damage and heavy burden. Immune biologic indicators must follow the development of infection and disease. One of immune biomarkers for TB diagnosis is Benefits of the T-SPOT.TB blood test. T-SPOT.TB is a unique blood test for TB testing, also known as the gamma-interferon test (IGRA). The T-SPOT.TB test in a pivotal clinical trial showed both sensitivity and quality exceeding 95% and reliability in all risk groups