Bacteriological and physiochemical parameters of groundwater were investigated in six (6) selected Communities of Gombe Metropolis with the core aim of examine effect of sewage on groundwater quality, water parameters such as: Bacteria, Temperature, pH, Total dissolve solid, Turbidity, Conductivity, Nitrate, Phosphate, Sodium, Hardness and Fluoride were analyzed. One way analysis of variance was employed to test the spatial variation in the concentration of aforementioned water parameters between low, medium and high density areas within the study area. T- test was also used compared the result obtained with WHO water quality acceptable standards. A total of 12 samples were collected from borehole water in high, medium and low density areas of the study area, afterward the water sample were analysed using culture media for bacteriology, while rest of the parameters were analysed in the laboratory. Culture media laboratory result indicates the present of bacteria in the water sample collected from all locations and confirmatory test reveals the types of bacteria as coliform bacteria. Analysis of variance conducted which shows that the concentration of coliformbacteria.