Improvement of Viral dsRNA Isolation from Infected Plants and Vector Nematodes
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To control the plant viral diseases, identification of the virus species is considered as a first step. Considering the stable structure of dsRNA in comparison to RNA molecules, dsRNA extraction is a promising tool to elucidate the viral genome sequence. Majority of the RNA viruses produce dsRNA segments as the copies of virus genome; these high molecular weight molecules can be employed in virus identification. To date, several efforts have been made to extract dsRNA from many different plant species. Developing a method charging less and completing fast has been investigated in this study.
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