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Quinoxaline is heterocyclic compound containing benzene ring and pyrazine ring. Pyrazine is water soluble and stable colourless compound. In quinoxaline, benzene ring is fused with diazines compounds. The pyrazine ring system is present in the fungal metabolite aspergillic acid and also in luciferin. Methoxy pyrazine are essential component of aroma of many fruits and vegetables such as capsicum and peas. Quinoxaline derivatives are well known in the pharmaceutical industry and have been shown to possess a broad spectrum of biological activities including antiviral, antibacterial, antiinflammatory, askinase inhibitors, anticancer and anthelmintic agents. Quinoxaline ring is a part of a number of antibiotics such as echinomycin, levomycin, and actinomycin, which are known to inhibit the growth of Gram-positive bacteria and are also active against various transplantable tumors and DNA cleaving agents.