This book contains the absolute information about how to perform In vitro fertilization technique in buffalo embryos that are collected from slaughter house derived ovaries. Since this was the work for the masters, the techniques mentioned are well standardized in the lab and fool proof in the species mentioned. However, there might be a variability in the techniques among different species but the idea would be the same. Alongside the detailed step by step description of the technique, there is also a succinct details of the downstream processes of molecular biology examinations of the lab derived blastocysts This should give the reader the idea as to how the journey from oocytes to embryos starts and can be taken further for our greater understanding until the molecular level.