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The study entitled "Effects of growth regulators on in vitro multiplication of Bacopa monnieri" was carried at Centre for Pant Biotechnology (CPB). The results obtained are summarized below: - The optimum time period of sterilization with no contamination and 100% survival for shoot tips is two minutes using 0.1% HgCl2. - The zero contamination with maximum survival rate was obtained when nodal segments were treated with 0.1 % mercuric chloride for three minutes. - Maximum regeneration in shoot tips and nodal segment was observed at minimum concentration (0. 25 mg/l) of auxins i.e. IBA and NAA. - The optimum concentration of IBA and NAA is 0.25 mg/l for each. - BAP and Kinetin were used alone maximum numbers of shoots were formed at the concentration of 1.0 mg/l. - Both the cytokinins (BAP and Kinetin) were used in combination the best results were obtained in BAP (1.0 mg/l) + Kinetin (0.2 mg/l) followed by BAP (0.2 mg/l) + Kinetin (1.0 mg/l). - Lower concentrations (1.0 & 0.2 mg/l) of cytokinins (BAP and Kinetin) either used alone or in combination shows good results.