This book has prepared from an experimental result of sugarcane tissue culture, it contains all paper preparation content. Tissue culture is a collection of techniques to grow plant cells, tissues and organs under sterile conditions. It is also known as in vitro culture; an example is micro propagation, in which a small piece of plant tissue is used to generate a whole plant. This technique is highly adopted in horticulture and forestry to allow rapid production of new, superior variety of offspring besides land/space and time saving through direct and indirect methods using different parts of plants such as shoot tip, leaf, meristem, buds, seed etc.