Capsicum (Capsicum annuum L.), commonly known as pepper, sweet pepper or bell pepper, belongs to the family Solanaceae and is one of the most important vegetable crops in the world. In spite of 400 different capsicum varieties found grown all over the world, the vulnerability of these genotypes consequent to the abiotic and biotic stresses has drastically restricted their yield potential and quality. Although, the conventional plant breeding combined with improved agricultural practices has upgraded capsicum crop production, quality and use remarkably, yet the restricted gene pool or range of organisms among which genes can be transferred, has placed a limit on this technology. Hence, gradually the biotechnology encompassing plant tissue culture and genetic engineering is becoming a functional tool of classical plant breeding to boost the crop improvement programs. Hence, the investigation on in vitro regeneration of capsicum was carried out and results were presented in this book