Md. Abu Zafur Al Munsur

In vitro regeneration of bitter gourd from seedling explants

Regeneration of bitter gourd

• Broschiertes Buch

Produktbeschreibung

A combination of 1.0 mgl-1 2,4-D and 1.0 mgl-1 BAP produced the highest callus frequency in nodal (93.75%), leaf (78.75%) and roots (75.00%) segments. In case of root tips 1.0 mgl-1 2,4-D combined with 0.5 mgl-1 BAP produced the highest callus (72.5%). Nodal segments required minimum number of 8.25 days while root tips required the maximum 32 days for callus induction. A combination of 2.5 mgl-1 BAP and 0.6 mgl-1 NAA produced the highest callus frequency (86.25%) in nodal segments followed by root segments (85.00%). Among the combinations 1.0 mgl-1 2, 4-D and 1.0 mgl-1 BAP was the most suitable for producing greenish friable callus and 2.0 or 2.5 mgl-1 BAP and 0.30 or 0.60 mgl-1 NAA was suitable for callus induction. A combination of 1.0 mgl-1 2, 4-D and 1.0 mgl-1 BAP exhibited 75.00% and 56.0% shoot direct regeneration from nodal segments and leaf segments, respectively. Nodal segments had 70% shoot regeneration via callus on medium with 2.0 mgl-1 BAP and 0.5 mg L-1 IBA + 0.2 mgl-1 GA3. Leaf segments and root tips had equal percentage (65.0) of shoot regeneration upon culture on medium with 2.0 mgl-1 BAP and 0.2 mgl-1 IAA.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 92
• Erscheinungstermin: 24. August 2011
• Englisch
• Abmessung: 220mm x 150mm x 6mm
• Gewicht: 155g
• ISBN-13: 9783845438641
• ISBN-10: 3845438649
• Artikelnr.: 33999723

Autorenporträt

Md. Abu Zafur Al Munsur has obtained his MS Degree from BAU, Mymensingh, Bangladesh. He has 23 published papers in different Journals. His interest area of research are plant tissue culture and plant biotechnology.