Tesfaye Disasa Bitema

In vitro regeneration of niger (Guizotia abyssinica Cass.)

using cotyledon and hypocotyl explants

• Broschiertes Buch

Produktbeschreibung

Callus induction and shoot formation from hypocotyls and cotyledons of Guizotia abyssinica Cass. has been achieved. Six to eight-day-old hypocotyl segments and cotyledons were cultured on MS medium containing different concentrations of NAA, IAA and BAP. Among the various concentrations tested, 0.5 mg/l NAA in combination with 1 mg/l BAP was found to be the best for maximum callus induction of hypocotyl explants. Furthermore, 2 mg/l IAA in combination with 1 mg/l BAP was the best for callus induction of cotyledonary explants. Highest percentage of shoot formation (60%) was obtained when cotyledons were cultured on medium supplemented with 3.0 mg/l IAA in combination with 1.0 mg/ l BAP. Maximum number of shoots per explant (20.3) was obtained from medium containing 0.1 mg/l NAA in combination with 1 mg/l BAP. The types of explant, growth regulator combinations and genotypes were showed significant effect on shoot regeneration. The elongated shoots were successfully rooted on mediasupplemented with IBA at a concentration of 0.5 mg/l. The shoots were established in soil where 65% of them survived. Morphologically aberrant plants were not observed.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Aufl.
• Seitenzahl: 72
• Englisch
• Abmessung: 220mm
• ISBN-13: 9783659168673
• ISBN-10: 365916867X
• Artikelnr.: 36040766

Autorenporträt

Got master degree in Applied Genetics in 2007 and bachelor degree in Applied Biology in 2004 from Addis Ababa University, Ethiopia. Currently doing PhD work at Addis Ababa University. Research interest: Molecular plant breeding and association mapping of drought tolerance traits.