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Rice (Oryza sativa L.) is one of the most important food crops of Southeast Asia, which feeds half of the world population. Anther culture is an efficient and convenient technique for rapid production of doubled haploids which are useful in crop breeding programs. The combination of different growth regulators facilitates callus induction on N6 medium and regeneration on MS medium. Highest callus induction frequency was recorded in growth regulator concentrations of 2, 4-D 2.0 mg/l + Kinetin 1.0 mg/l (34.54%) followed by in growth regulator concentrations 2, 4-D 1.0 mg/l + NAA 2.0 mg/l + Kinetin 0.5 mg/l (33.38%). Highest regeneration frequency was recorded in growth regulator concentrations of Kinetin 0.5 mg/l + BAP 2.0 mg/l + NAA 1.0 mg/l (69.44%) followed by in growth regulator concentrations of Kinetin 2.0 mg/l + BAP 1.0 mg/l + NAA 1.0 mg/l (40.00%). This is clearly indicated that type of growth regulator along with its concentration plays important role in callus induction and regeneration in japonica rice varieties.