Levofloxacin is a synthetic second-generation bactericidal fluoroquinolone with a wide spectrum of activity. It's used to treat bacterial infections such as acute bacterial sinusitis, influenza, urinary tract infections, chronic prostatitis, and some forms of gastroenteritis. The analysis of levofloxacin is important because it's high dose causes adverse effects to the human body. The chromatography and spectroscopic techniques is used to estimate the levofloxacin drug.