This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated inflammatory disease models affecting barrier organs to the environment; the skin, the lung, and the intestinal and oral mucosa. The fourth section illustrates inflammatory disease models of the brain, joints, and vasculature. Written in the highly successful Methods in Molecular Biology…mehr
This volume presents a broad selection of cutting-edge methods and tools that will enable the reader to investigate the multi-faceted manifestations of inflammation. Inflammation: Methods and Protocols is divided into four sections: the first three sections describe protocols investigating immune-mediated inflammatory disease models affecting barrier organs to the environment; the skin, the lung, and the intestinal and oral mucosa. The fourth section illustrates inflammatory disease models of the brain, joints, and vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and practical, Inflammation: Methods and Protocols aims to inspire the experienced investigator and the young experimenter alike to disentangle the fascinating process of inflammation.
Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models.- Hapten-specific T cell-mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate.- Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry.- Visualization of the T Cell Response in Contact Hypersensitivity.- Ultraviolet Radiation-induced Immunosuppression - Induction of Regulatory T cells.- Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model.- Xenotransplantation Model of Psoriasis.- A Mouse Model for Atopic Dermatitis using Topical Application of Vitamin D3 or of its Analog MC9039 Particle Bombardment of Ex-vivo Skin to Deliver DNA and Express Proteins.- Mouse Models of Asthma.- Subcutaneous and Sublingual Immunotherapy in a Mouse Model of Allergic Asthma.- Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse.-Induction and Analysis of Bronchus-associated Lymphoid Tissue.- Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodes.- Isolation and Identification of Intestinal Myeloid Cells.- Monitoring and Modulation of Inducible Foxp3+ Regulatory T-cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon.- Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria.- Analysis of Leukocytes in Oral Mucosal Tissues.- Optimized Mouse Models for Liver Fibrosis.- Monitoring of Chemically Induced Colitis.- Oxazolone-induced Intestinal Inflammation in Adult Zebrafish.- High Dimensional Cytometry of Central Nervous System Leukocytes During Inflammation.- Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System.- Investigating the Lymphatic Drainage of the Brain: Essential Skills and Tools.- Quantitative Assessment of Cerebral Basement Membranes using Electron Microscopy.- Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish.- Experimental Arthritis Mouse Models Driven by Adaptive and/or Innate Inflammation.- Pain Relief in Nonhuman Primate Models of Arthritis.- Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis.- Assessment of Vascular Dysfunction and Inflammation Induced by Angiotensin II in Mice.
Reproducibility Issues: Avoiding Pitfalls in Animal Inflammation Models.- Hapten-specific T cell-mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate.- Monitoring Skin Dendritic Cells in Steady State and Inflammation by Immunofluorescence Microscopy and Flow Cytometry.- Visualization of the T Cell Response in Contact Hypersensitivity.- Ultraviolet Radiation-induced Immunosuppression - Induction of Regulatory T cells.- Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model.- Xenotransplantation Model of Psoriasis.- A Mouse Model for Atopic Dermatitis using Topical Application of Vitamin D3 or of its Analog MC9039 Particle Bombardment of Ex-vivo Skin to Deliver DNA and Express Proteins.- Mouse Models of Asthma.- Subcutaneous and Sublingual Immunotherapy in a Mouse Model of Allergic Asthma.- Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse.-Induction and Analysis of Bronchus-associated Lymphoid Tissue.- Messenger RNA Sequencing of Rare Cell Populations in the Lung and Lung-Draining Lymph Nodes.- Isolation and Identification of Intestinal Myeloid Cells.- Monitoring and Modulation of Inducible Foxp3+ Regulatory T-cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon.- Isolation and Flow Cytometry Analysis of Innate Lymphoid Cells from the Intestinal Lamina Propria.- Analysis of Leukocytes in Oral Mucosal Tissues.- Optimized Mouse Models for Liver Fibrosis.- Monitoring of Chemically Induced Colitis.- Oxazolone-induced Intestinal Inflammation in Adult Zebrafish.- High Dimensional Cytometry of Central Nervous System Leukocytes During Inflammation.- Isolation of Microglia and Immune Infiltrates from Mouse and Primate Central Nervous System.- Investigating the Lymphatic Drainage of the Brain: Essential Skills and Tools.- Quantitative Assessment of Cerebral Basement Membranes using Electron Microscopy.- Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish.- Experimental Arthritis Mouse Models Driven by Adaptive and/or Innate Inflammation.- Pain Relief in Nonhuman Primate Models of Arthritis.- Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis.- Assessment of Vascular Dysfunction and Inflammation Induced by Angiotensin II in Mice.
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