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In this work, the effect of the strain path change on the mechanical behavior of magnesium alloy AZ31 was investigated by sequential uniaxial tensile tests performed at room temperature. The pre-strain was carried out up to a value of 0.07 in the rolling direction (RD) of the sheet. From the pre-deformed samples, specimens at 0º, 15º, 30º, 45º, 60º, 75º and 90º from the RD were cut and reload in uniaxial tensile test. The influence of the dislocation microstructure and the crystallographic texture developed during the plastic deformation on the mechanical behavior of the material during the pre-strain and reloading was analyzed by transmission electron microscopy, x-ray diffraction and numerical simulations using the polycrystalline visco-plastic self-consistent model. The results showed that the AZ31 alloy presented a strong mechanical anisotropic behavior during reloading, characterized by a decrease of the flow stress with the angle between the two deformation paths. Accordingto the calculations, this behavior is associated with a decrease of the average Taylor factor due to the development of a (0001)101