A field experiment was carried out at Junagadh Agricultural University, Junagadh during Kharif season of the year 2013-14 and 2014-15 to study the integrated weed management in Bt cotton and determination of their persistence through bioassay technique". The results of the experiment revealed that next to the weed free treatment, application of oxyfluorfen 0.180 kg ha-1 PE fb IC & HW at 30 & 60 DAS enhanced growth parameters viz., plant height, monopodial & sympodial branches per plant, dry matter per plant, yield attributes viz., number of bolls per plant, single boll weight, number of seeds per boll, seed cotton weight per plant, 100-seed weight, lint yield, lint index and ginning percentage along with seed cotton and stalk yield, quality parameters viz., fibre length, fibre fineness, fibre strength, oil content and oil yield, and reduced weeds population and dry weight of weeds over other treatments. This treatment also inhibited depletion of nutrients (NPK) by weeds and increased uptake of nutrient by the crop and ultimately increased post-harvest soil fertility.