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This current laboratory manual is based on the original manuscript written by the late Dr. Michael S Parker (1961-2003). Dr. Parker worked diligently in designing and coordinating the microbiology laboratories at University of Memphis. In this new edition, we substitute most bacteria used in the original manual with bacteria that are classified as Biosafety Level 1 organisms to improve laboratory safety. New chapters on the use of internet- and computer- assisted gene analysis are now added to provide students with hands on experience in this new area of microbiology and bioinformatics. This manual is supported, in part, by a TAF grant from the University of Memphis. The Editor also thanks Ms Leigh McFarland for her careful review and her valuable comments on this manual.