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Mungbean (Vigna radiata (L.) Wilczek) is one of the important pulse crop, primarily grown for food in India. During the survey, occurrence of Mungbean Yellow Mosaic Virus (MYMV) in mungbean was noticed in serious proportion causing heavy losses in Navsari, Surat and Valsad district. Cultivar GM-4 and K-851 was found more severely affected at the flowering stage during summer season. Considering the seriousness of the problem, the present investigation was carried out virus transmission and detection of virus.