This volume contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolomics which aim to address this dilemma and to help pinpoint new treatment strategies. Chapters detail the generation of several models and methods for assessing health and provide researchers potential approaches for reversing or minimizing effects of disease. In addition, important information on disease mechanisms is provided, as each method is described in the context of a specific disease or…mehr
This volume contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolomics which aim to address this dilemma and to help pinpoint new treatment strategies. Chapters detail the generation of several models and methods for assessing health and provide researchers potential approaches for reversing or minimizing effects of disease. In addition, important information on disease mechanisms is provided, as each method is described in the context of a specific disease or therapeutic area. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications aim is to provide insights into the latest ideas and technologies .enabling progress in this field.
Nutritional programming effects on development of metabolic disorders in later life.- Effects of Prenatal Nutrition and the Role of the Placenta in Health and Disease.- Developmental Origins of Stress and Psychiatric Disorders.- Proteomic Studies of Psychiatric Disorders.- Developmental Origins of Breast Cancer: A Paternal Perspective.- Point-of-care Testing and Personalized Medicine for Metabolic Disorders.- Pregnancy and Lactation a Window of Opportunity to Improve Individual Health.- Utility of Small Animal Models of Developmental Programming.- Generation of Maternal Obesity Models in Studies of Developmental Programming in Rodents.- Generation of the Maternal Low Protein Rat Model for Studies of Metabolic Disorders.- Investigation of Paternal Programming of Breast Cancer Risk in Female Offspring in Rodent Models.- Studies of Isolated Peripheral Blood Cells as a Model of Immune Dysfunction.- Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders.- Assessment of Placental Transport Function in Studies of Disease Programming.- Assessment of Fatty Liver in Models of Disease Programming.- Capillary Blood Sampling from the Finger.- Physical Activity Assessment in Clinical Studies of Substance use Disorder.- Cardiopulmonary Exercise Testing.- Cardiovascular Assessment in Human Research.- Nutritional Programming Effects on the Immune System.- Small RNA Sequencing - A Technique for miRNA Profiling.- Pulse-chase Biosynthetic Radiolabelling of Pancreatic Islets to Measure Beta Cell Function.- Rapid and Easy Protocol for Quantification of Next Generation Sequencing Libraries.- Telomere length analysis - A Tool for Dissecting Ageing Mechanisms in Developmental Programming.- Qualitative and quantitative NMR Approaches in Blood Serum Lipidomics.- In vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models.- Experimental Model of Skeletal Muscle Laceration in Rats.- Neuropsychiatric Sequelae of Early Nutritional Modifications: A Beginner's Guide to Behavioural Analysis.- Hyperlocomotion Test for Assessing Behavioural Disorders.- 2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation.- Mass Spectrometry Profiling of Pituitary Glands.- Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation.- Time Resolved Fluorescence Assays for Quantification of Insulin Precursors in Plasma and Serum.- Identification of Neural Stem Cell Biomarkers by Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) Mass Spectrometry.- Lab-on-a-chip Device for Rapid Measurement of Vitamin D Levels.- Kidney Smartphone Diagnostics.- A User-friendly App for Blood Coagulation Disorders.- Hormonal Smartphone Diagnostics.
Nutritional programming effects on development of metabolic disorders in later life.- Effects of Prenatal Nutrition and the Role of the Placenta in Health and Disease.- Developmental Origins of Stress and Psychiatric Disorders.- Proteomic Studies of Psychiatric Disorders.- Developmental Origins of Breast Cancer: A Paternal Perspective.- Point-of-care Testing and Personalized Medicine for Metabolic Disorders.- Pregnancy and Lactation a Window of Opportunity to Improve Individual Health.- Utility of Small Animal Models of Developmental Programming.- Generation of Maternal Obesity Models in Studies of Developmental Programming in Rodents.- Generation of the Maternal Low Protein Rat Model for Studies of Metabolic Disorders.- Investigation of Paternal Programming of Breast Cancer Risk in Female Offspring in Rodent Models.- Studies of Isolated Peripheral Blood Cells as a Model of Immune Dysfunction.- Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders.- Assessment of Placental Transport Function in Studies of Disease Programming.- Assessment of Fatty Liver in Models of Disease Programming.- Capillary Blood Sampling from the Finger.- Physical Activity Assessment in Clinical Studies of Substance use Disorder.- Cardiopulmonary Exercise Testing.- Cardiovascular Assessment in Human Research.- Nutritional Programming Effects on the Immune System.- Small RNA Sequencing - A Technique for miRNA Profiling.- Pulse-chase Biosynthetic Radiolabelling of Pancreatic Islets to Measure Beta Cell Function.- Rapid and Easy Protocol for Quantification of Next Generation Sequencing Libraries.- Telomere length analysis - A Tool for Dissecting Ageing Mechanisms in Developmental Programming.- Qualitative and quantitative NMR Approaches in Blood Serum Lipidomics.- In vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models.- Experimental Model of Skeletal Muscle Laceration in Rats.- Neuropsychiatric Sequelae of Early Nutritional Modifications: A Beginner's Guide to Behavioural Analysis.- Hyperlocomotion Test for Assessing Behavioural Disorders.- 2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation.- Mass Spectrometry Profiling of Pituitary Glands.- Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation.- Time Resolved Fluorescence Assays for Quantification of Insulin Precursors in Plasma and Serum.- Identification of Neural Stem Cell Biomarkers by Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) Mass Spectrometry.- Lab-on-a-chip Device for Rapid Measurement of Vitamin D Levels.- Kidney Smartphone Diagnostics.- A User-friendly App for Blood Coagulation Disorders.- Hormonal Smartphone Diagnostics.
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