Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.
Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.
Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USAHenry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA
Inhaltsangabe
Preface
Daniel L. Minor Jr. and Henry M. Colecraft
Structure/function relationships
1. Correlating ion channel structure and function
Crina Nimigean
2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM
Alexander Sobolevsky
3. Methods to study phosphoinositide regulation of ion channels
Tibor Rohacs
4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies
Sudha Chakarpani
5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography
Anna Moroni
Structural and chemical methods to assess channel dynamics
6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy
Simon Scheuring
7. Crosslinking for gating motions in GluRs
Andrew Plested
8. Single molecule FRET methodology for investigating glutamate receptors
Vasanthi Jayaraman
9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues
Murali Prakriya
Channel reconstitution and studies in bilayers
10. A quantitative flux assay for the study of Cl- channels and transporters
Alessio Accardi
11. Ion channels reconstitution in lipid bilayers
Eleonora Zakharian
12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers
Gehard Thiel
Methods to assess oligomerization
13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture
