IR imaging of Fiber/Cell interactions for the study of Asbestos effect - Yao, Seydou
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Interaction of asbestos fibers with human cells cause lung diseases such as asbestosis and mesothelioma, whose severity is directly dependent on the cellular phenotypes involved. Details of this process remain largely unknown because of the chemical heterogeneity and morphology of the fibers and the requirement of organic-inorganic analytical means. Indeed, the toxicity of these fibers depends not only on their dimensions, but also on their habit and surface chemistry. Therefore, there are multiple fiber-cell interactions and phenomena making challenging their interpretation. Because of their…mehr

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Produktbeschreibung
Interaction of asbestos fibers with human cells cause lung diseases such as asbestosis and mesothelioma, whose severity is directly dependent on the cellular phenotypes involved. Details of this process remain largely unknown because of the chemical heterogeneity and morphology of the fibers and the requirement of organic-inorganic analytical means. Indeed, the toxicity of these fibers depends not only on their dimensions, but also on their habit and surface chemistry. Therefore, there are multiple fiber-cell interactions and phenomena making challenging their interpretation. Because of their non invasiveness, their ability to investigate at a cellular level and the possibility to probe molecular function, spectroscopic methods as FTIR and Raman spectroscopy are suitable techniques to evaluate these fiber cells interactions. We developed a method to analyze the effect of the interactions between inorganic materials (asbestos fibers) and organic materials (cells). Thus, the use ofspectroscopic techniques allowed a further insight into the mechanism of defence of single living cells interacting with asbestos fiber.
Autorenporträt
Dr Seydou YAO is a Research Officer based in Centre for Nanohealth (CNH) in Swansea University. He's part of the Reproductive Biological Group with the purpose of the understanding of the human embryo implantation. To perform it, he combines molecular biological techniques with Atomic Force Microscopy, infrared or high screening technique as INCELL