Melanie Hager

Isotopic fractionation during microbial decane degradation

Assessing bioremediation at hydrocarbon polluted sites with carbon and hydrogen isotopes

• Broschiertes Buch

Produktbeschreibung

Groundwater from a diesel fuel-contaminated site in Vienna, Austria, was treated in pilot-scale subsurface flow constructed wetlands. The study aims at quantifying the contribution of biodegradation to the total removal of diesel compounds by means of compound-specific isotope analysis (CSIA). This innovative approach requires a constant isotope fractionation during the microbial transformation reaction which leads to an enrichment of the heavy isotopes, i. e. 13C and 2H, in the remaining pollutant. Filter material and/or groundwater was incubated in laboratory microcosms and the aerobic degradation of n-decane, which was chosen as model compound for diesel hydrocarbons, was monitored. Isotope fractionation was absent in water saturated microcosms because only minor n-decane biodegradation took place. During n-decane biodegradation in moist filter material, on the other hand, small but variable isotopic fractionation factors were found. The different experimental set-ups give indications how mass transfer limitations of hydrophobic molecules might mask isotopic fractionation and the consequences for field applications of CSIA are discussed in detail.

Produktdetails

• Verlag: AV Akademikerverlag
• Seitenzahl: 192
• Erscheinungstermin: 7. September 2016
• Englisch
• Abmessung: 220mm x 150mm x 12mm
• Gewicht: 272g
• ISBN-13: 9783330502697
• ISBN-10: 333050269X
• Artikelnr.: 46151156

Autorenporträt

Hager Melanie, born 1986/02/01. Education: Environmental engineering and water management, terminated 2016/04/27 at the University of Applied Life Sciences, Vienna.Master Thesis: 'Determination of the 13C/12C and 2H/1H isotopic fractionation factor during microbial degradation of decane: a microcosm study'