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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. A knockout moss is a moss plant in which one or more specific genes are deleted or inactivated by gene targeting. After deletion of a gene, the knockout moss has lost the trait encoded by this gene. Thus, the function of this gene can be inferred. This scientific approach is called reverse genetics as the scientist wants to unravel the function of a specific gene. In classical genetics the scientist starts with a phenotype of interest and searches for the gene that…mehr

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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. A knockout moss is a moss plant in which one or more specific genes are deleted or inactivated by gene targeting. After deletion of a gene, the knockout moss has lost the trait encoded by this gene. Thus, the function of this gene can be inferred. This scientific approach is called reverse genetics as the scientist wants to unravel the function of a specific gene. In classical genetics the scientist starts with a phenotype of interest and searches for the gene that causes this phenotype. Knockout-mosses are relevant for basic research in biology as well as in biotechnology. The targeted deletion or alteration of genes relies on the integration of a short DNA-strand at a specific and predictable position into the genome of the host cell. This DNA-strand must be engineered in such a way that both ends are identical to this specific gene locus. This is a prerequisite for being efficiently integrated via homologous recombination.